Files
dashboard-app/app.R
T

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138 KiB
R

library(shiny)
library(shinydashboard)
library(shinyjs)
library(shinyAce)
library(shinydashboard)
library(shinycssloaders)
library(shinyBS)
library(purrr)
library(gslnls)
library(tidyverse)
library(ggplot2)
library(reshape2)
library(openxlsx)
library(DT)
library(ggpubr)
library(gridExtra)
library(drc)
library(twopartm)
library(car)
library(dplyr)
#### reactive values ----
Dat <- reactiveValues()
REP <- reactiveValues()
#### functions ----
dilFUN2 <- function(cs_,dils,Faktor) {
av <- cs_
dils_av <- dils_av
dils_avsc <- dils_av*Faktor
dils2 <- dils_avsc+av
dilfactors <- 1/exp(dils2-lag(dils2))
return(dilfactors)
}
plot_f <- function(dat, sigmoid,det_sig, TransFlag=F) {
CORdat <- cor(dat[,1],dat[,ncol(dat)])
#browser()
all_l <- melt(data.frame(dat), id.vars="log_dose", variable.name="replname", value.name = "readout")
isRef <- rep(c(1,0),1,each=nrow(all_l)/2)
isSample <- rep(c(0,1),1,each=nrow(all_l)/2)
all_l2 <- cbind(all_l, isRef, isSample)
#browser()
if(is.null(det_sig)) {
startlist <- list(a=sigmoid[1], b=sigmoid[5],cs=sigmoid[7],
d=sigmoid[3],r=sigmoid[8])
} else {
startlist <- list(a=det_sig[5], b=det_sig[1],cs=det_sig[7],
d=det_sig[3],r=det_sig[7] - det_sig[8])
}
mr <- gsl_nls(fn = readout ~ a+(d-a)/(1+exp(b*((cs-r*isSample)-log_dose))),
data=all_l,
start=startlist,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
s_mr <- tryCatch({
s_mr <- summary(mr)
},
error = function(err) {
s_mr <- NULL
})
a <- s_mr$coefficients["a",1]
b <- s_mr$coefficients["b",1]
cs <- s_mr$coefficients["cs",1]
d <- s_mr$coefficients["d",1]
r <- s_mr$coefficients["r",1]
log_dose <- unique(all_l$log_dose)
seq_x <- seq(min(log_dose),max(log_dose),0.1)
SAMPLE <- a+(d-a)/(1+exp(b*((cs-r)-seq_x)))
REF <- a+(d-a)/(1+exp(b*((cs)-seq_x)))
if (is.null(det_sig)) {
SAMPLEtrue <- sigmoid[2] + (sigmoid[4] -sigmoid[2])/(1+exp(sigmoid[6]*((sigmoid[7]-sigmoid[8]-seq_x))))
REFtrue <- sigmoid[1] + (sigmoid[3] -sigmoid[1])/(1+exp(sigmoid[5]*((sigmoid[7]-seq_x))))
} else {
SAMPLEtrue <- det_sig[4] + (det_sig[6] -det_sig[4])/(1+exp(det_sig[2]*(det_sig[8]-seq_x)))
REFtrue <- det_sig[3] + (det_sig[5] -det_sig[3])/(1+exp(det_sig[1]*(det_sig[7]-seq_x)))
}
#browser()
pl_df <- cbind(seq_x, SAMPLE, REF, SAMPLEtrue, REFtrue)
all_l2$readout[all_l2$readout < 0] <- 0.01
all_l2$readouttrans <- log(all_l2$readout)
slopeEC50 <- b*(d-a)/4
Intercept <- a+(d-a)/2-b*(d-a)/4*cs
#browser()
Xbendl3 <- cs-(1.31696/b)
Xbendu3 <- cs+(1.31696/b)
XbendlT <- cs-r-(1.31696/b)
XbenduT <- cs-r+(1.31696/b)
XasymplS <- cs-(3/b)
XasympuS <- cs+(3/b)
XasymplT <- cs-r-(3/b)
XasympuT <- cs-r+(3/b)
bendpoints <- c(bendREF_lower = round(Xbendl3,3), bendREF_upper=round(Xbendu3,3),
bendSAMPLE_lower = round(XbendlT,3), bendSAMPLE_upper=round(XbenduT,3),
asympREF_lower = round(XasymplS,3), asympREF_upper=round(XasympuS,3),
asympSAMPLE_lower = round(XasymplT,3), asympSAMPLE_upper=round(XasympuT,3))
Dat$bendpoints <- bendpoints
Dat$cfordils <- cs
p <- ggplot(all_l2, aes(x=log_dose, y=readout, color=factor(isRef))) +
geom_point(shape=factor(isRef), alpha=0.8) +
labs(title = paste("restricted 4pl"),
color="product") +
scale_color_manual(labels=c("test","reference"), values=c("#C2173F","#4545BA")) +
scale_shape_manual(labels=c("test","reference")) +
theme_bw() +
theme(axis.text = element_text(size=14))
p2 <- p + geom_line(data=as.data.frame(pl_df), aes(x=seq_x, y=SAMPLE), color="#C2173F",
inherit.aes = F) +
geom_line(data=as.data.frame(pl_df), aes(x=seq_x, y=REF), color="#4545BA",
inherit.aes = F) +
geom_line(data=as.data.frame(pl_df), aes(x=seq_x, y=SAMPLEtrue), color="#C2173F", linetype=2, alpha=0.4,
inherit.aes = F) +
geom_line(data=as.data.frame(pl_df), aes(x=seq_x, y=REFtrue), color="#4545BA", linetype=2, alpha=0.4,
inherit.aes = F) +
geom_vline(xintercept=c(Xbendl3, Xbendu3), col="#4545BA",linetype=2) +
geom_vline(xintercept=c(XbendlT, XbenduT), col="#C2173F",linetype=2) +
geom_vline(xintercept=c( XasymplS, XasympuS), col="#4545BA55",linetype=2) +
geom_vline(xintercept=c(XasymplT, XasympuT), col="#C2173F55",linetype=2) +
annotate("text", x=cs, y=a+(d-a)/2, label="0", size=5) +
geom_abline(slope = slopeEC50, intercept = Intercept) +
theme(legend.position="none")
Dat$p2 <- p2
# transformed plots
p_rt <- ggplot(all_l2, aes(x=log_dose, y=readouttrans, color=factor(isRef))) +
geom_point(shape=factor(isRef), alpha=0.8) +
labs(title = paste("restricted transformed 4pl"), color="product") +
scale_color_manual(labels=c("test","reference"), values=c("#C2173F","#4545BA")) +
theme_bw()
mrt <- gsl_nls(fn = readouttrans ~ a+(d-a)/(1+exp(b*((cs-r*isSample)-log_dose))),
data=all_l2,
start=startlist,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
s_mrt <- summary(mrt)
a_trans <- s_mrt$coefficients["a",1]
b_trans <- s_mrt$coefficients["b",1]
cs_trans <- s_mrt$coefficients["cs",1]
d_trans <- s_mrt$coefficients["d",1]
r_trans <- s_mrt$coefficients["r",1]
XbendlTrans <- cs_trans-(1.31696/b_trans)
XbenduTrans <- cs_trans+(1.31696/b_trans)
XbendlTransT <- cs_trans-r_trans-(1.31696/b_trans)
XbenduTransT <- cs_trans-r_trans+(1.31696/b_trans)
bendpointsTRANS <- c(bendREF_lower = round(XbendlTrans,3), bendREF_upper=round(XbenduTrans,3),
bendSAMPLE_lower = round(XbendlTransT,3), bendSAMPLE_upper=round(XbenduTransT,3))
Dat$bendpointsTRANS <- bendpointsTRANS
SAMPLEtrans <- a_trans+(d_trans-a_trans)/(1+exp(b_trans*((cs_trans-r_trans)-seq_x)))
REFtrans <- a_trans+(d_trans-a_trans)/(1+exp(b_trans*((cs_trans)-seq_x)))
pl_df_trans <- cbind(seq_x, SAMPLEtrans, REFtrans)
p_rt2 <- p_rt + geom_line(data=as.data.frame(pl_df_trans), aes(x=seq_x, y=SAMPLEtrans), color="#C2173F",
inherit.aes = F) +
geom_line(data=as.data.frame(pl_df_trans), aes(x=seq_x, y=REFtrans), color="#4545BA",
inherit.aes = F) +
geom_vline(xintercept=c(XbendlTrans, XbenduTrans), col="#4545BA",linetype=2) +
geom_vline(xintercept=c(XbendlTransT, XbenduTransT), col="#C2173F",linetype=2) +
theme(legend.position = "none", axis.text=element_text(size=14))
#browser()
if(is.null(det_sig)) {
startlistmu <- list(as=sigmoid[1], bs=sigmoid[5],cs=sigmoid[7],
ds=sigmoid[3], at=sigmoid[2], bt=sigmoid[6],
dt=sigmoid[4],r=sigmoid[8])
} else {
startlistmu <- list(as=det_sig[5], bs=det_sig[1],cs=det_sig[7],
ds=det_sig[3],at=det_sig[6], bt=det_sig[2],
dt=det_sig[4],r=det_sig[7] - det_sig[8])
}
tryCatch({
mu <- gsl_nls(fn = readout ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmu,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(msg){
return(0) })
Sum_u <- tryCatch({ summary(mu) },
error=function(msg){
return(0) })
#browser()
#if (is.null(det_sig)) {
ast <- Sum_u$coefficients["as",1]
ate <- Sum_u$coefficients["at",1]
bst <- Sum_u$coefficients["bs",1]
bte <- Sum_u$coefficients["bt",1]
cst <- Sum_u$coefficients["cs",1]
cte <- Sum_u$coefficients["cs",1]-Sum_u$coefficients["r",1]
dst <- Sum_u$coefficients["ds",1]
dte <- Sum_u$coefficients["dt",1]
# } else {
# ast <- det_sig[5]
# ate <- det_sig[6]
# bst <- det_sig[1]
# bte <- det_sig[2]
# cst <- det_sig[7]
# cte <- det_sig[8]
# dst <- det_sig[3]
# dte <- det_sig[4]
# }
REFu <- ast + (dst-ast)/(1+exp(bst*(cst-seq_x)))
SAMPLEu <- ate + (dte-ate)/(1+exp(bte*(cte-seq_x)))
pl_df2 <- cbind(seq_x, SAMPLEu, REFu)
#browser()
pu <- ggplot(all_l2, aes(x=log_dose, y=readout, color=factor(isRef))) +
geom_point() +
labs(title="unrestricted 4_pl-Model", color="product") +
scale_color_manual(labels = c("test","reference"), values=c("#C2173F","#4545BA")) +
theme_bw()
pu2 <- pu + geom_line(data=as.data.frame(pl_df2), aes(x=seq_x, y=SAMPLEu),
color="#C2173F", inherit.aes = F) +
geom_line(data=as.data.frame(pl_df2), aes(x=seq_x, y=REFu),
color="#4545BA", inherit.aes = F,
show.legend = F)
pu2_ <- pu2 +
theme(legend.position = "none", axis.text = element_text(size=14))
putrans <- ggplot(all_l2, aes(x=log_dose, y=readouttrans, color=factor(isRef))) +
geom_point() +
labs(title="unrestricted transformed 4_pl-Model", color="product") +
scale_color_manual(labels = c("test","reference"), values=c("#C2173F","#4545BA")) +
theme_bw()
unrestr_trans <- drm(readouttrans ~ exp(log_dose), isSample, data=all_l2, fct=LL.4(),
pmodels=data.frame(isSample, isSample,isSample,isSample))
Sum_ut <- summary(unrestr_trans)
ast_t <- Sum_ut$coefficients[3,1]
ate_t <- Sum_ut$coefficients[4,1]
bst_t <- Sum_ut$coefficients[1,1]
bte_t <- Sum_ut$coefficients[2,1]
cst_t <- log(Sum_ut$coefficients[7,1])
cte_t <- log(Sum_ut$coefficients[8,1])
dst_t <- Sum_ut$coefficients[5,1]
dte_t <- Sum_ut$coefficients[6,1]
REFu_trans <- ast_t + (dst_t-ast_t)/(1+exp(bst_t*(seq_x-cst_t)))
SAMPLEu_trans <- ate_t + (dte_t-ate_t)/(1+exp(bte_t*(seq_x-cte_t)))
pl_df2u_t <- cbind(seq_x, SAMPLEu_trans, REFu_trans)
pu2_t <- putrans + geom_line(data=as.data.frame(pl_df2u_t), aes(x=seq_x, y=SAMPLEu_trans),
color="#C2173F", inherit.aes = F) +
geom_line(data=as.data.frame(pl_df2u_t), aes(x=seq_x, y=REFu_trans),
color="#4545BA", inherit.aes = F,
show.legend = F)
pu3_t <- pu2_t
if (TransFlag) grid.arrange(p_rt2,pu3_t, nrow=1) else grid.arrange(p2,pu2_, nrow=1)
}
Calc_DilRes <- function(as=3, bs=1, cs=-4, ds=10, at=3, bt=1, dt=10,r=0.0001,ct=cs-r,
sd_fac=0.1, gt=1, gs=1, log_conc,
heteroNoise=FALSE, noDilSeries, noDils) {
yAxfac <- (ds-as)
log_dose <- log_conc
isRef <- rep(c(1,0),1,each=length(log_conc)*noDilSeries)
isSample <- rep(c(0,1),1,each=length(log_conc)*noDilSeries)
#browser()
av <- as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(ct-log_dose)))
if (heteroNoise) {
# heterosc noise
ro_jit <- matrix(unlist(map(av, function(x) x+rnorm(1,0,x*sd_fac/100))), nrow=noDils, ncol=noDilSeries*2)
} else {
# homosc noise
ro_jit <- matrix(unlist(map(av, function(x) x+rnorm(1,0,sd_fac*yAxfac/100))), nrow=noDils, ncol=noDilSeries*2)
}
ro_jit <- abs(ro_jit)
ro_jit2 <- cbind(ro_jit, log_dose)
if (noDilSeries==3) {
colnames(ro_jit2) <- c("R_dil1","R_dil2","R_dil3","T_dil1","T_dil2","T_dil3", "log_dose")
} else {
colnames(ro_jit2) <- c("R_dil1","R_dil2","T_dil1","T_dil2", "log_dose")
}
return(ro_jit2)
}
LinPotTab <- function(circles, Lim, PureErrFlag) {
circ_ABl <- circles
circ_Al <- circ_ABl[circ_ABl$isSample ==1,]
circ_Al <- circ_ABl[circ_ABl$isSample ==0,]
# restr CSSI model
modAB <- lm(readout ~ log_dose + isSample, circ_ABl)
coeffs <- modAB$coefficients
SU_modAB <- tryCatch({
SU_modAB <- summary(modAB)
}, error = function(msg) {
return(NA)
})
# Intercept diff/slope modAB
linPot <- exp(modAB$coefficients[3]/modAB$coefficients[2])
if(PureErrFlag) {
FitAnova <- anova(lm(readout ~ factor(log_dose)*isSample, circ_ABl))
meanPureErr <- FitAnova[4,3]
DFsPure <- FitAnova[4,1]
VCOV <- vcov(modAB)
V_V <- VCOV/SU_modAB$sigma^2
VCOVpure <- V_V*meanPureErr
SEsPure <- sqrt(diag(V_V)*meanPureErr)
}
log_pot_delta <- deltaMethod(modAB, "isSample/log_dose")
if (PureErrFlag) {
V_ <- log_pot_delta$SE^2/SU_modAB$sigma^2
V_p <- V_*meanPureErr
potDeltaPureSE <- sqrt(V_p)
CI_log_low <- log_pot_delta$Estimate - qt(0.975, DFsPure)*potDeltaPureSE
CI_log_up <- log_pot_delta$Estimate + qt(0.975, DFsPure)*potDeltaPureSE
} else {
CI_log_low <- log_pot_delta$Estimate - qt(0.975, df.residual(modAB))*log_pot_delta$SE
CI_log_up <- log_pot_delta$Estimate + qt(0.975, df.residual(modAB))*log_pot_delta$SE
}
#browser()
ExpLinPot <- exp(c(log_pot_delta$Estimate, CI_log_low, CI_log_up))
if (ExpLinPot[2]*100>Lim[[9]] & ExpLinPot[3]*100<Lim[[10]]) test_potCI <- 0 else test_potCI <- 1
# Rel pot CI
relLinpotCI <- ExpLinPot/linPot*100
pottab <- cbind(round(linPot*100,3), round(ExpLinPot[2]*100,3), round(ExpLinPot[3]*100,3),
round(test_potCI,3), round(relLinpotCI[2],3),round(relLinpotCI[3],3))
colnames(pottab) <- c("Potency","lower 95%CI", "upper 95%CI", "test_result", "lowerRel95%CI","upperRel95%CI")
return(pottab)
}
ANOVAlintests <- function(ro_new, circles, Lim, PureErrFlag) {
all_l <- melt(data.frame(ro_new), id.vars="log_dose", variable.name = "replname", value.name = "readout")
isRef <- rep(c(1,0),1,each=nrow(all_l)/2)
isSample <- rep(c(0,1),1,each=nrow(all_l)/2)
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
all_lA <- all_l[all_l$isSample == 1,] # TEST
all_lB <- all_l[all_l$isSample == 0,] # REF
circ_ABl <- circles
circ_Al <- circ_ABl[circ_ABl$isSample ==1,]
circ_Bl <- circ_ABl[circ_ABl$isSample ==0,]
# restr CSSI model
modAB <- lm(readout ~ log_dose + isSample, circ_ABl)
# unrestr with interact term SSSI
modABu <- lm(readout ~ log_dose + isSample + log_dose*isSample, circ_ABl)
modA <- lm(readout ~ log_dose + isSample, circ_Al)
modB <- lm(readout ~ log_dose + isSample, circ_Bl)
log_pot_delta <- deltaMethod(modAB, "isSample/log_dose")
CI_log_low <- log_pot_delta$Estimate - qt(0.975, df.residual(modAB))*log_pot_delta$SE
CI_log_up <- log_pot_delta$Estimate + qt(0.975, df.residual(modAB))*log_pot_delta$SE
ExpLinPot <- exp(c(log_pot_delta$Estimate, CI_log_low, CI_log_up))
if (ExpLinPot[2]*100>Lim[9] & ExpLinPot[3]*100>Lim[10]) test_potCI <- 0 else test_potCI <- 1
su_mod <- summary(modAB)$coefficients
su_mod2 <- cbind(data.frame(parameter = c("intercept REF","slope REF","intercepts diff.")), su_mod)
su_modU <- summary(modABu)$coefficients
su_modU2 <- cbind(data.frame(parameter = c("intercept REF","slope REF","intercepts diff.","slope difference")), su_modU)
uCI_SloDiff <- su_modU[4,1] + qt(0.975,8)*su_modU[4,2]
lCI_SloDiff <- su_modU[4,1] - qt(0.975,8)*su_modU[4,2]
SlopeDiffCI <- c(su_modU[4,1], lCI_SloDiff,uCI_SloDiff)
lenCirc <- nrow(circ_ABl)
dfTreat <- 3
dfPrep <- 1
dfReg <- 1
dfnonP <- 1
dfRMSE <- c(lenCirc-3-1)
dfTotal <- lenCirc-1
dfPureE <- lenCirc-6
dfNonLin <- dfRMSE-dfPureE
RSS <- sum(resid(lm(readout ~ log_dose*isSample, circ_ABl))^2)
MSE <- RSS/dfRMSE
SSE <- sum(resid(lm(readout ~ factor(log_dose)*isSample, circ_ABl))^2)
MSpure <- SSE/dfPureE
if (PureErrFlag) {
FitAnova <- anova(lm(readout ~ factor(log_dose)*isSample, circ_ABl))
meanPureErr <- FitAnova[4,3]
SU_modAB <- tryCatch({
SU_modAB <- summary(modAB)
}, error = function(msg) {
return(NA)
})
if (length(SU_modAB)>1) s_modABcoeffs <- summary(modAB)$coefficients
DFsPure <- FitAnova[4,1]
VCOV <- vcov(modAB)
V_V <- VCOV/SU_modAB$sigma^2
VCOVpure <- V_V*meanPureErr
SEsPure <- sqrt(diag(V_V)*meanPureErr)
su_mod2[,3] <- SEsPure
su_mod2[,4] <- su_mod2[,2]/su_mod2[,3]
su_mod2[,5] <- 2*(1-pt(abs(su_mod[,4]), FitAnova[4,1]))
s_mu <- summary(modABu)$coefficients
SU_modABu <- summary(modABu)
VCOVu <- vcov(modABu)
V_Vu <- VCOVu/SU_modABu$sigma^2
SEsPureU <- sqrt(diag(V_Vu)*meanPureErr)
su_modU2[,3] <- SEsPureU
su_modU2[,4] <- su_modU2[,2]/su_modU2[,3]
su_modU2[,5] <- 2*(1-pt(abs(su_modU2[,4]), FitAnova[4,1]))
uCI_SloDiffP <- su_modU[4,1] + qt(0.975,8)*SEsPureU[4]
lCI_SloDiffP <- su_modU[4,1] - qt(0.975,8)*SEsPureU[4]
SlopeDiffCI <- c(su_modU[4,1], lCI_SloDiffP,uCI_SloDiffP)
SSRes <- SSE
dfRes <- dfPureE
} else {
SSRes <- RSS
dfRes <- dfRMSE
}
# treatment
SStreat <- print(sum((predict(lm(readout ~ factor(log_dose)*isSample, circ_ABl))-mean(circ_ABl$readout))^2))
F_treat <- (SStreat/dfTreat)/(SSRes/dfRes)
# Preparation
SSprep <- print(sum((predict(lm(readout ~ isSample, circ_ABl))-mean(circ_ABl$readout))^2))
F_prep <- (SSprep/dfTreat)/(SSRes/dfRes)
# Regression
# ANOVA tape II SS of regression
SSreg <- Anova(lm(readout ~log_dose + isSample, circ_ABl))[1,1]
# Non-parallelism
# diff of RSS of restricted and unrestricted model
SSnonpar <- sum(resid(modAB)^2) - sum(resid(modABu)^2)
F_nonpar <- SSnonpar/(sum(resid(lm(readout ~ factor(log_dose)*isSample, circ_ABl))^2)/(lenCirc-4))
# non-linearity
SSnonlin <- sum((predict(modABu)-predict(lm(readout ~ as.factor(log_dose)*isSample, circ_ABl)))^2)
# = RSS-SSE
# Total SS
SStot <- sum((circ_ABl$readout-mean(circ_ABl$readout))^2)
# Significance of R^2 F-ratio
# MSR/MSE
# sample A
F_R2_A <- sum((predict(lm(readout ~ log_dose+ I(log_dose^2), circ_Al)) - mean(predict(modA)))^2 - (predict(modA) - mean(circ_Al$readout))^2)/
(sum((predict(lm(readout ~ log_dose+ I(log_dose^2), circ_Al)) - circ_Al$readout)^2)/(nrow(circ_Al)-3))
pFR2_A <- round(pf(F_R2_A,1,6),4)
# sample B
F_R2_B <- sum((predict(lm(readout ~ log_dose+ I(log_dose^2), circ_Bl)) - mean(predict(modB)))^2 - (predict(modB) - mean(circ_Bl$readout))^2)/
(sum((predict(lm(readout ~ log_dose+ I(log_dose^2), circ_Bl)) - circ_Bl$readout)^2)/(nrow(circ_Bl)-3))
pFR2_B <- round(pf(F_R2_B,1,6),4)
# sign of non-lin with pure error: MSSnonlin/MSSE
F_nonlin <- (SSnonlin/2)/(SSE/dfPureE)
# sign of slope
F_slope_B <- sum((predict(modB) - mean(circ_Bl$readout))^2)/(sum((circ_Bl$readout - predict(modB))^2)/(nrow(circ_Bl)-2))
F_slope_A <- sum((predict(modA) - mean(circ_Al$readout))^2)/(sum((circ_Al$readout - predict(modA))^2)/(nrow(circ_Al)-2))
# F-test on regression: MSSreg/MSSE
if (is.na(F_nonlin)) F_nonlin <- 0
if (F_nonlin > 0) {
p_F_nonlin <- round(pf(F_nonlin,2,dfPureE, lower.tail = F),5)
} else { p_F_nonlin <- "SSnonlin neg or 0"; }
# significances
F_regr <- (SSreg/1)/(SSRes/dfRes)
p_F_regr <- round(pf(F_regr,1,dfRes, lower.tail = F),3)
p_F_treat <- round(pf(F_treat,3,dfRes, lower.tail = F),3)
p_F_prep <- round(pf(F_prep,1,dfRes, lower.tail = F),3)
p_F_slope_A <- round(pf(F_slope_A,1,(nrow(circ_Al)-2), lower.tail = F),3)
p_F_slope_B <- round(pf(F_slope_B,1,(nrow(circ_Bl)-2), lower.tail = F),3)
p_F_nonp <- round(pf(F_nonpar,1,dfRes, lower.tail = F),3)
p_F_LoF <- p_F_nonlin
res_tab_lin <- data.frame(test = c("F-test on sign. of regression", "F_test on non-lin",
"F-test on R^2 A","F_test on R^2 B",
"F-test on slope A","F-test on slope B",
"F-test on non-parallelism","F-test on preparation"),
test_results = c(ifelse(p_F_regr<0.05,0,1),ifelse(p_F_nonlin<0.05,1,0),
ifelse(pFR2_A<0.05,1,0),ifelse(pFR2_B<0.05,1,0),
ifelse(p_F_slope_A<0.05,0,1),ifelse(p_F_slope_B<0.05,0,1),
ifelse(p_F_nonp<0.05,1,0),ifelse(p_F_prep<0.05,0,1)),
estimate = c(p_F_regr, p_F_nonlin,pFR2_A,pFR2_B,p_F_slope_A,
p_F_slope_B,p_F_nonp,p_F_prep),
Source = c("Treatment","Preparation","Regression","Non-parallelism",
"Resid Error","Non-linearity","Pure error", "Total"),
df = c(dfTreat,1,1,1,dfRMSE,2,dfPureE,lenCirc-1),
SumSquares = c(round(SStreat,5),round(SSprep,5),round(SSreg,5),
round(SSnonpar,5),round(RSS,5),round(SSnonlin,5),
round(SSE,5),round(SStot,5)),
MS = c(round(SStreat/dfTreat,5),round(SSprep,5),round(SSreg,5),
round(SSnonpar,5),round(RSS/dfRMSE,5),round(SSnonlin/2,5),
round(SSE/dfPureE,5),round(SStot/dfTotal,5)),
"F-value" = c(round(F_treat,5), round(F_prep,5),round(F_regr,5),
round(F_nonpar,5),"",round(F_nonlin,5),"",""),
"p-value" = c(p_F_treat, p_F_prep, p_F_regr, p_F_nonp, "", p_F_LoF, "",""))
RET <- list(res_tab_lin, su_modU2, SlopeDiffCI, su_mod2)
return(RET)
}
pot4plFUNC <- function(ro_new, PureErrFlag) {
all_l <- melt(data.frame(ro_new), id.vars="log_dose", variable.name="replname", value.name = "readout")
isRef <- rep(c(1,0),1,each=nrow(all_l)/2)
isSample <- rep(c(0,1),1,each=nrow(all_l)/2)
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
all_l$readout[all_l$readout < 0] <- 0.01
all_l$readouttrans <- log(all_l$readout)
CORdat <- cor(ro_new[,1],ro_new[,ncol(ro_new)])
if (CORdat<0) SLOPE <- -1 else SLOPE <- 1
startlist <- list(a=min(ro_new[,2]), b=SLOPE, d=max(ro_new[,2]), cs=mean(all_l$log_dose),r=0)
tryCatch({
mr <- gsl_nls(fn = readout ~ a+(d-a)/(1+exp(b*(cs-r*isSample-log_dose))),
data=all_l,
start=startlist,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
startlistmu <- list(as=max(ro_new[,2]), bs=SLOPE, ds=min(ro_new[,2]), cs=mean(all_l$log_dose),
at=max(ro_new[,2]), bt=SLOPE, dt=min(ro_new[,2]), r=0)
tryCatch({
mu <- gsl_nls(fn = readout ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmu,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
if (!PureErrFlag) {
pot_est <- exp(confintd(mr, "r", method="asymptotic"))
potU_est <- exp(confintd(mu, "r", method="asymptotic"))
} else {
FitAnova <- anova(lm(readout ~ factor(Conc)*isSample, all_l))
meanPureErr <- FitAnova[4,3]
SU_mr <- tryCatch({
SU_mr <- summary(mr)
}, error = function(msg) {
return()
})
browser()
if (length(SU_mr)>1) {
s_mr <- SU_mr$coefficients
} else { SU_mr <- rep(NA,5) }
VCOV <- vcov(mr)
V_V <- VCOV/SU_mr$sigma^2
SEsPure <- sqrt(diag(V_V)*meanPureErr)
pot_est <- c(exp(s_mr['r',1]),exp(s_mr['r',1]-qt(0.975,nrow(all_l)-5)*SEsPure['r']),
exp(s_mr['r',1]+qt(0.975,nrow(all_l)-5)*SEsPure['r']))
# unrestricted
s_mu <- summary(mu)$coefficients
SU_mu <- summary(mu)
VCOVu <- vcov(mu)
V_Vu <- VCOVu/SU_mu$sigma^2
SEsPureU <- sqrt(diag(V_Vu)*meanPureErr)
potU_est <- c(exp(s_mu['r',1]),exp(s_mu['r',1]-qt(0.975,nrow(all_l)-8)*SEsPureU['r']),
+ exp(s_mu['r',1]+qt(0.975,nrow(all_l)-8)*SEsPureU['r']))
} # PureErrFlag
startlistmr_log <- list(a=max(all_l$readouttrans), b=SLOPE, d=min(all_l$readouttrans), cs=mean(all_l$log_dose),r=0)
tryCatch({
mr_log <- gsl_nls(fn = readouttrans ~ a+(d-a)/(1+exp(b*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmr_log,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
startlistmu_log <- list(as=max(ro_new[,2]), bs=SLOPE, ds=min(ro_new[,2]), cs=mean(all_l$log_dose),
at=max(ro_new[,2]), bt=SLOPE, dt=min(ro_new[,2]), r=0)
tryCatch({
mu_log <- gsl_nls(fn = readouttrans ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmu_log,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
pot_est_log <- exp(confintd(mr_log, "r", method="asymptotic"))
potU_est_log <- exp(confintd(mu_log, "r", method="asymptotic"))
colnames(pot_est_log) <- c("estimate","lowerCI2","upperCI")
colnames(potU_est_log) <- c("estimate","lowerCI2","upperCI")
#browser()
su_mr_log <- summary(mr_log)
Dat$RMSE_Rlog <- su_mr_log$sigma
su_mu_log <- summary(mu_log)
Dat$RMSE_Ulog <- su_mu_log$sigma
Dat$up_lowAslog <- su_mu_log$coefficients[1,1] - su_mu_log$coefficients[4,1]
potALL <- rbind(pot_est, potU_est, pot_est_log, potU_est_log)
potALL2 <- cbind(c("restricted","unrestricted","ln-transformed restr","ln-transformed unrestr"), potALL)
return(potALL2)
}
ParamCI_F <- function(xt,xs,se_xt, se_xs, CoVarlog,DFs, Conf=0.975) {
log_xs <- log(abs(xs))
log_xt <- log(abs(xt))
var_log_xs <- (se_xs/xs)^2 # approximate variance of log(bs)
var_log_xt <- (se_xt/xt)^2
se_log_ratio <- sqrt(var_log_xs + var_log_xt) #-2*CoVarlog)
lower_log_ratio <- log_xt-log_xs - qt(Conf,DFs)*se_log_ratio
upper_log_ratio <- log_xt-log_xs + qt(Conf,DFs)*se_log_ratio
ci_ratio <- exp(c(lower_log_ratio, upper_log_ratio))
return(ci_ratio)
}
tests_FUNC <- function(ro_new, Lim, PureErrFlag) {
#browser()
all_l <- melt(data.frame(ro_new), id.vars="log_dose", variable.name="replname", value.name = "readout")
isRef <- rep(c(1,0),1,each=nrow(all_l)/2)
isSample <- rep(c(0,1),1,each=nrow(all_l)/2)
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
all_l$readout[all_l$readout < 0] <- 0.01
tryCatch({
pot <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(1,1,1,isSample))
},
error = function(msg){
return(0) })
compParm(pot, "c",display=T)
ED50 <- ED(pot,c(50), interval="delta")
PotEst <- ED50[1,1]/ED50[2,1]
potAll <- EDcomp(pot, percVec=c(50,50), interval="delta", display=FALSE)
potAll2 <- potAll[1:3]
CORro <- cor(ro_new[,1], ro_new[,ncol(ro_new)])
if (CORro<0) SLOPE <- -1 else SLOPE <- 1
startlist <- list(a=max(ro_new[,2]), b=SLOPE, d=min(ro_new[,2]), cs=mean(all_l$log_dose),r=0)
tryCatch({
mr <- gsl_nls(fn = readout ~ a+(d-a)/(1+exp(b*(cs-r*isSample-log_dose))),
data=all_l,
start=startlist,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(msg){
return(0) })
startlistmu <- list(as=max(ro_new[,2]), bs=SLOPE, ds=min(ro_new[,2]), cs=mean(all_l$log_dose),
at=max(ro_new[,2]), bt=SLOPE, dt=min(ro_new[,2]), r=0)
tryCatch({
mu <- gsl_nls(fn = readout ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmu,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(msg){
return(0) })
smu <- tryCatch({ summary(mu) },
error=function(msg){
return(0) })
POTr_CI <- potAll2[2:3]
FitAnova <- anova(lm(readout ~ factor(Conc)*isSample, all_l))
# pure error
pureSS <- FitAnova[4,2]
pureSS_df <- FitAnova[4,1]
meanPureErr <- FitAnova[4,3]
vcovMU <- vcov(mu)
V_V <- vcovMU/smu$sigma^2
SEsPure <- sqrt(diag(V_V)*meanPureErr)
VCOVpure <- V_V*meanPureErr
DFsPure <- FitAnova[4,1]
testPOTr <- logical()
if (POTr_CI[1]*100>Lim[[9]] & POTr_CI[2]*100<Lim[[10]] ) testPOTr <- 0 else testPOTr <- 1
potU <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(isSample, isSample,isSample,isSample))
potAllU <- EDcomp(potU, percVec=c(50,50), interval="delta", display=FALSE)
potAllU2 <- potAllU[1:3]
sum_potU <- summary(potU)
coeffs <- potU$coefficients
coeffs[1] <- ifelse(CORro<0, -coeffs[1], coeffs[1])
coeffs[2] <- ifelse(CORro<0, -coeffs[2], coeffs[2])
names(coeffs) <- c("bs","bt","ds","dt","as","at","cs","ct")
e_c_ref <- coeffs[7]
e_c_test <- coeffs[8]
coeffs[7:8] <- log(coeffs[7:8])
test_c <- logical()
if((potAllU2[2] >Lim[[9]]/100 & potAllU2[3] <Lim[[10]]/100)) test_c <- 0 else test_c <- 1
#### ANOVA ----
noConc <- length(unique(all_l$Conc))
nofitted <- noConc
AnovaDFs <- c(nofitted-1,1,3,nofitted-4-1,nrow(all_l)-nofitted, nofitted,nrow(all_l)-2*nofitted,nrow(all_l)-1)
SStreat <- round(sum((predict(potU)-mean(all_l$readout))^2),5)
SSregr <- round(sum((predict(pot)-mean(all_l$readout))^2),5)
# non-parallelism
SSnonparall <- round(sum(resid(pot)^2)-sum(resid(potU)^2),5)
SSprep <- round(sum((predict(lm(readout ~ isSample, all_l))-mean(all_l$readout))^2),5)
RSS <- round(sum(potU$predres[,2]^2),5)
RSS_df <- AnovaDFs[5]
MSEunr <- RSS/RSS_df
RMSEunr <- sqrt(RSS/RSS_df)
# Pure Err
FitAnova <- anova(lm(readout ~ factor(Conc)*isSample, all_l))
SSE <- sum(resid(lm(readout ~ factor(Conc)*isSample, all_l))^2) # =FitAnova[4,2]
SSE_df <- FitAnova[4,1]
PureMSE <- SSE/SSE_df
RMSE_pure <- sqrt(PureMSE)
## non-lin = LoF
if (PureErrFlag) { ERR <- PureMSE; ERR_df <- SSE_df } else { ERR <- MSEunr; ERR_df <- RSS_df }
SSnonlin <- sum((predict(lm(readout ~ factor(Conc)*isSample, all_l))-predict(potU))^2)
LoF_df <- FitAnova[1,1]+FitAnova[2,1]
F_regr <- (SSregr/AnovaDFs[3])/ERR
p_F_regr <- round(pf(F_regr, AnovaDFs[3], ERR_df, lower.tail = F),5)
if (ncol(ro_new)<4) F_nonlin <- 0 else F_nonlin <- (SSnonlin/AnovaDFs[6])/ERR
if (F_nonlin > 0) {
p_F_nonlin <- round(pf(F_nonlin, AnovaDFs[6], ERR_df, lower.tail = F),5)
} else { p_F_nonlin <- "SSnonlin neg or single dilutions" }
test_a <- test_b <- test_d <- test_ad <- logical()
RSS_r <- round(sum(pot$predres[,2]^2),5)
MSE_r <- RSS_r/(nrow(all_l)-5)
RMSE_r <- round(sqrt(MSE_r),6)
Dat$RMSE_r <- RMSE_r
Dat$RMSE_pure <- RMSE_pure
Dat$RMSE_unr <- round(RMSEunr,6)
#browser()
## EQ test on lower As diff
ds <- coeffs["ds"]
dt <- coeffs["dt"]
lAs_diff <- (dt-ds)
uCI_laDiff <- lAs_diff+qt(0.975,df.residual(mu))*sqrt(sum_potU$coefficients[3,2]^2+sum_potU$coefficients[4,2]^2)
lCI_laDiff <- lAs_diff-qt(0.975,df.residual(mu))*sqrt(sum_potU$coefficients[3,2]^2+sum_potU$coefficients[4,2]^2)
if (uCI_laDiff < Lim[[2]] & lCI_laDiff > Lim[[1]]) test_la_diff <- 0 else test_la_diff <- 1
#### EQ test on upper asymptote ratio ----
as <- coeffs["as"]
at <- coeffs["at"]
uAsRatio <- compParm(potU, "a","/",display=F)
uAsCI <- c(uAsRatio[1]-qt(0.975,RSS_df)*uAsRatio[2], uAsRatio[1]+qt(0.975,RSS_df)*uAsRatio[2])
#browser()
ds <- smu$coefficients["ds",1]
dt <- smu$coefficients["dt",1]
if (PureErrFlag) se_ds <- sqrt(VCOVpure["ds","ds"]) else se_ds <- smu$coefficients["ds",2]
if (PureErrFlag) se_dt <- sqrt(VCOVpure["dt","dt"]) else se_dt <- smu$coefficients["dt",2]
if (PureErrFlag) CoVarlog_d <- VCOVpure["dt","ds"] else CoVarlog_d <- vcovMU["dt","ds"]
if (PureErrFlag) DFs <- DFsPure else DFs <- nrow(all_l)-noConc
uAsCI2 <- ParamCI_F(dt,ds,se_dt, se_ds,CoVarlog_d, DFs, Conf=0.9975)
if (uAsCI2[1] > Lim[[7]] & uAsCI2[2] < Lim[[8]]) test_a <- 0 else test_a <- 1
estUppA <- round(at/as,5)
Dat$uAsCI <- uAsCI2
#### EQ test on slope ratio ----
bs <- coeffs["bs"]
bt <- coeffs["bt"]
slopeRatio <- compParm(potU, "b","/",display=F)
slopeCI <- c(slopeRatio[1,1]-qt(0.975,RSS_df)*slopeRatio[1,2], slopeRatio[1,1]+qt(0.975,RSS_df)*slopeRatio[1,2])
bs <- smu$coefficients["bs",1]
bt <- smu$coefficients["bt",1]
if (PureErrFlag) se_bs <- sqrt(VCOVpure["bs","bs"]) else se_bs <- smu$coefficients["bs",2]
if (PureErrFlag) se_bt <- sqrt(VCOVpure["bt","bt"]) else se_bt <- smu$coefficients["bt",2]
if (PureErrFlag) CoVarlog_b <- VCOVpure["bt","bs"] else CoVarlog_b <- vcovMU["bt","bs"]
slopeCI2 <- ParamCI_F(bt,bs,se_bt, se_bs,CoVarlog_b, DFs, Conf=0.975)
if (slopeCI2[1] > Lim[[5]] & slopeCI2[2] < Lim[[6]]) test_b <- 0 else test_b <- 1
estUppA <- round(at/as,5)
Dat$slopeRatioCI <- slopeCI
#### EQ test on lower As ratio ----
lAsRatio <- compParm(potU, "d","/",display=F)
slopeCI <- c(lAsRatio[1,1]-qt(0.975,RSS_df)*lAsRatio[1,2], lAsRatio[1,1]+qt(0.975,RSS_df)*lAsRatio[1,2])
as <- smu$coefficients["as",1]
at <- smu$coefficients["at",1]
if (PureErrFlag) se_as <- sqrt(VCOVpure["as","as"]) else se_as <- smu$coefficients["as",2]
if (PureErrFlag) se_at <- sqrt(VCOVpure["at","at"]) else se_at <- smu$coefficients["at",2]
if (PureErrFlag) CoVarlog_a <- VCOVpure["at","as"] else CoVarlog_a <- vcovMU["at","as"]
lAsCI2 <- ParamCI_F(at,as,se_at, se_as,CoVarlog_a, DFs, Conf=0.975)
if (lAsCI2[1] > Lim[[3]] & lAsCI2[2] < Lim[[4]]) test_d <- 0 else test_d <- 1
estLowA <- round(at/as,5)
Dat$lAsCI <- lAsCI2
#### EQtest on ratio of As difference ----
AsDiffRatio <- (at-dt)/(as-ds)
at_dt <- (at-dt)
as_ds <- (as-ds)
se_ds_asPure <- sqrt(VCOVpure["as","as"]+VCOVpure["ds","ds"]-2*VCOVpure["as","ds"])
se_dt_atPure <- sqrt(VCOVpure["at","at"]+VCOVpure["dt","dt"]-2*VCOVpure["at","dt"])
se_ds_asRMSE <- sqrt(vcovMU["as","as"]+vcovMU["ds","ds"]-2*vcovMU["as","ds"])
se_dt_atRMSE <- sqrt(vcovMU["at","at"]+vcovMU["dt","dt"]-2*vcovMU["at","dt"])
if (PureErrFlag) se_ds_as <- se_ds_asPure else se_ds_as <- se_ds_asRMSE
if (PureErrFlag) se_dt_at <- se_dt_atPure else se_dt_at <- se_dt_atRMSE
AsDiffCI2 <- ParamCI_F(at_dt,as_ds,se_dt_at, se_ds_as,CoVarlog=0, DFs, Conf=0.975)
if (AsDiffCI2[1] > Lim[[11]] & AsDiffCI2[2] < Lim[[12]]) test_ad <- 0 else test_ad <- 1
estLowA <- round(at/as,5)
Dat$up_lowAs <- abs(ds-as)
lowerCIlowerA <- lAsCI2[1]; lowerCIupperA <- uAsCI2[1]; upperCIlowerA <- lAsCI2[2]; upperCIupperA <- uAsCI2[2]
test_lowA <- test_d; test_uppA <- test_a
#browser()
res_tab <- data.frame(test= c("F-test on sign. of regression*",
"EQ test on lower asymptotes difference",
"EQ test ratio of lower asymptotes",
"EQ test ratio of Hill slopes",
"EQ test ratio of upper asymptotes",
"F-test on non-linearity*",
"EQ test ratio of asymptote difference",
"geom. rel. CI restr. model",
"geom. rel. CI unrestr. model"),
test_results = c(ifelse(p_F_regr<0.05,0,1), test_la_diff, test_lowA, test_b, test_uppA,
ifelse(p_F_nonlin>1,1, ifelse(p_F_nonlin<0.05,1,0)), test_ad,
testPOTr, test_c),
estimate = c(round(p_F_regr, 3), round(lAs_diff, 5),
estLowA, round(bs/bt,5), estUppA, p_F_nonlin,
round(at_dt/as_ds, 5), round(potAll2[1]*100,2),round(potAllU2[1]*100,2)),
lower_limit = c("-",Lim[[1]],Lim[[3]],Lim[[5]],Lim[[7]],"-",Lim[[11]],Lim[[9]],Lim[[9]]),
upper_limit = c("-",Lim[[2]],Lim[[4]],Lim[[6]],Lim[[8]],"-",Lim[[12]],Lim[[10]],Lim[[10]]),
lower_CI = c(RMSE_r, round(lCI_laDiff,3), round(lAsCI2[1],5), round(slopeCI2[1],5),
round(uAsCI2[1],5), "-", round(AsDiffCI2[1],5), round(potAll2[2],2), round(potAllU2[2],2)),
upper_CI = c(RMSE_pure, round(uCI_laDiff,3), round(lAsCI2[2],5), round(slopeCI2[2],5),
round(uAsCI2[2],5), "-", round(AsDiffCI2[2],5), round(potAll2[3],2), round(potAllU2[3],2))
)
return(res_tab)
}
ANOVA4plUnresfunc <- function(ro_new, sigmoid) {
all_l <- melt(data.frame(ro_new), id.vars="log_dose", variable.name="replname", value.name = "readout")
all_len <- nrow(all_l)
isRef <- rep(c(1,0),1,each=all_len/2)
isSample <- rep(c(0,1),1,each=all_len/2)
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
all_l$readout[all_l$readout < 0] <- 0.01
pot <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(1,1,1,isSample))
potU <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(isSample, isSample,isSample,isSample))
SStreat <- round(sum((potU$predres[,1] - mean(all_l$readout))^2),5)
SStreat_df <- length(unique(all_l$log_dose))-1
SSregr <- round(sum((predict(pot)-mean(all_l$readout))^2),5)
## Non-parallel
SSnonparallel <- round(sum(resid(pot)^2) - sum(resid(potU)^2),5)
## Preparation
SSprep <- round(sum((predict(lm(readout ~ isSample, all_l)) - mean(all_l$readout))^2),5)
## Resid Err
RSS <- round(sum(potU$predres[,2]^2),5)
RSS_df <- nrow(all_l)-SStreat_df-1
FitAnova <- anova(lm(readout ~ factor(Conc)*isSample, all_l))
# PureErr
SSE <- FitAnova[4,3]
SSE_df <- FitAnova[4,1]
# Non-Linearity
SSnonlin <- round(sum((predict(lm(readout ~ factor(Conc)*isSample, all_l)) - predict(potU))^2),4)
LoF_df <- FitAnova[1,1]+FitAnova[2,1]
## Total
SStot <- round(sum((all_l$readout -mean(all_l$readout))^2),5)
MSE <- RSS/RSS_df
noConc <- length(unique(all_l$Conc))
AnovaDFs <- c(noConc-1, 1,3,noConc-4-1, nrow(all_l)-noConc, noConc, nrow(all_l)-noConc-noConc, nrow(all_l)-1)
p_SStreat <- round(pf((SStreat/AnovaDFs[1])/MSE, AnovaDFs[1],RSS_df, lower.tail = F),3)
p_SSprep <- round(pf((SSprep/AnovaDFs[2])/MSE, AnovaDFs[2],RSS_df, lower.tail = F),3)
p_SSregr <- round(pf((SSregr/AnovaDFs[3])/MSE, AnovaDFs[3],RSS_df, lower.tail = F),3)
p_SSnonp <- round(pf((SSnonparallel/AnovaDFs[4])/MSE, AnovaDFs[3],RSS_df, lower.tail = F),3)
p_SSLoF <- round(pf((SSnonlin/LoF_df)/(SSE/SSE_df), LoF_df,SSE_df, lower.tail = F),5)
ANOVAtab <- data.frame(Source = c("Treatment","Preparation","Regression",
"Non-Parallelism","Residual Error","Non-linearity",
"Pure Error","Total"),
DF = AnovaDFs,
SumSquares = c(SStreat, SSprep,SSregr, SSnonparallel,
RSS, SSnonlin,SSE, SStot),
MeanSquares = c(round(SStreat/AnovaDFs[1],3), SSprep, round(SStreat/AnovaDFs[3],3),round(SSnonparallel/AnovaDFs[4],3),
round(MSE,5), round(SSnonlin/LoF_df,5), round(SSE/SSE_df,5),""),
"F-value" = c(round((SStreat/AnovaDFs[1])/MSE,5), round((SSprep/AnovaDFs[2])/MSE,5),
round((SSregr/AnovaDFs[3])/MSE,5),round((SSnonparallel/AnovaDFs[4])/MSE,5),
"",round((SSnonlin/LoF_df)/(SSE/SSE_df),5),"",""),
"p_value" = c(round(p_SStreat,3), p_SSprep, round(p_SSregr,3), p_SSnonp,"",p_SSLoF,"","")
)
return(ANOVAtab)
}
perConcTab <- function(ro_new, noDilSeries) {
Reftab <- ro_new[,c(1:noDilSeries)]
Testtab <- ro_new[,c((noDilSeries+1):(2*noDilSeries))]
tReftab <- t(Reftab)
colnames(tReftab) <- round(ro_new[,ncol(ro_new)],5)
avs <- apply(tReftab,2,mean)
sds <- apply(tReftab,2,sd)
cv <- sds/avs*100
tReftab2 <- rbind(tReftab, avs,sds,cv)
tTesttab <- t(Testtab)
colnames(tTesttab) <- round(ro_new[,ncol(ro_new)],5)
avs_test <- apply(tTesttab,2,mean)
sds_test <- apply(tTesttab,2,sd)
cv_test <- sds_test/avs_test*100
tTesttab2 <- rbind(tTesttab, avs_test,sds_test,cv_test)
concTab <- rbind(tReftab2, tTesttab2)
return(concTab)
}
divFUN <- function(x,Div,N,res,noDil) {
N <- N+1
y <- x/Div
res <- c(res,y)
if (N==noDil) { return(res) }
divFUN(y,Div,N,res,noDil)
}
#### ui ----
ui <- dashboardPage(
dashboardHeader(title = "Plateflow"),
dashboardSidebar(
sidebarMenu(
img(src="logo.png", width=230),
menuItem("Home", tabName="home", icon=icon("home")),
menuItem("Data template", tabName = "template", icon=icon("table"),
menuSubItem( tags$li(a("EXCEL File", target="self",href="TestFile.xlsx")))
),
# menuItem("User Manual /Validation", tabName = "manual", icon=icon("book"), # tabName here and in dashboard body need to be identical
# menuSubItem(icon = NULL, tags$li(a("Document", target="self",href="UserManual.pdf")))
# ),
menuItem("EXCEL upload", tabName="Dataupload", icon=icon("magnet", lib="glyphicon")),
menuItem("4PL + report", tabName="fourPL", icon=icon("chart-line", lib="font-awesome")),
#menuItem("XLSX diagnostics", tabName="XLdiagn", icon=icon("chart-bar", lib="font-awesome")),
menuItem("Linear regression + report", tabName="pla", icon=icon("pencil", lib="glyphicon")),
menuItem("Wizard", tabName="wizard", icon=icon("chart-column", lib="font-awesome")),
menuItem("Documentation", tabName="documentation", icon=icon("chart-area", lib="font-awesome"))
),
tags$footer(HTML(paste(tags$strong(tags$u("InnerAnalytics")), paste(rep("&nbsp",9), collapse=""),
"Developer:", paste(rep("&nbsp",9), collapse=""),
"Host on:", paste(rep("&nbsp",9), collapse=""),
"Version:")),
align="left", style=
"position:fixed; bottom:0;width=100%; background: #FFC337BB; font-family: Times New Roman; font-size:100%;
padding: 5px; color:#4545BA; box-sizing: border-box; z-index: 1000;")),
dashboardBody(
fluidPage(
tabItems(
tabItem(tabName = "home", htmlOutput("homePage")),
tabItem(tabName = "Dataupload", uiOutput("Dataupload")),
tabItem(tabName = "fourPL", uiOutput("fourPL")),
#tabItem(tabName = "XLdiagn", uiOutput("XLdiagn")),
tabItem(tabName = "pla", uiOutput("pla")),
tabItem(tabName = "wizard", uiOutput("wizard")),
tabItem(tabName = "documentation", uiOutput("docu"))
)
)
), skin="blue"
)
#### server ----
server <- function(input, output, session) {
ReportParS <- reactiveValues()
IPReportParS <- reactiveValues()
#### renderUIs ----
output$homePage <- renderUI({
navbarPage("Home",
tabPanel("Introduction",
tags$img(src="logo.png", class="adv_logo"),
h4("Introduction to the bioassay software"),
tags$mark("linear regression"), br(),
column(4,
tags$table(id="dose-table",
numericInput("lEACdiffla","lower EAC for diff. of LA", -0.175, step=0.001),
numericInput("uEACdiffla","upper EAC for diff. of LA", 0.189, step=0.001),
numericInput("lEACratiola","lower EAC ratio of LAs", 0.005, step=0.001),
numericInput("uEACratiola","upper EAC for ratio of LAs", 100, step=1),
numericInput("lEACratioSlope","lower EAC for ratio of slopes", 0.55, step=0.01),
numericInput("uEACratioSlope","upper EAC for ratio of slopes", 1.84, step=0.1),
numericInput("lEACratioua","lower EAC for ratio of UAs", 0.75, step=0.1),
numericInput("uEACratioua","upper EAC for ratio of UAs", 1.33, step=0.1),
numericInput("lowerPot","lower EAC for potency", 75, step=1),
numericInput("upperPot","upper EAC for potency", 133, step=1),
numericInput("lEACratioAdiff","lower EAC of ratio of asymptote differences", 0.75, step=0.01),
numericInput("uEACratioAdiff","upper EAC of ratio of asymptote differences", 1.33, step=0.01)
))
),
tabPanel("Documentation",
h4("Introduction "),
h4("Information on dilution setting"),
"(for details see:", a(href="ADONIS.pdf","Whitepaper", download=NA, target="_blank"),")",tags$br(),
"Bend points are calculated according to following formula:",
withMathJax(" $$bp_{1/2} = \\pm\\frac{1.31696}{Hill's slope}$$")),
tabPanel("Configuration",
verbatimTextOutput("sessioninfo"))
)
})
output$Dataupload <- renderUI({
navbarPage(title="Information",
tabPanel(title = "Real data",
tabsetPanel(
tabPanel("Data input",
column(3,
#img(src="Screenshot.png", width=200),
box(title = "Upload", status="warning",solidHeader = T, width=12, "Please upload your EXCEL file here",
fileInput("iFile",'',accept=".xlsx")),
uiOutput(outputId = "sheetName"),
"For data format in the EXCEL file see Data template",
"If no data are uploaded, the settings to the right are used for calculations.",
tags$head(tags$style(HTML("label {font-size:80%;margin-bottom: 3px;margin-top: 3px;}"))),
div(checkboxInput("PureErr", "Should pure error be used for calculation of CIs?", FALSE),
style = "font-size: 24px !important;color: #C2173F"),
checkboxGroupInput("selectedSSTs", "Which suitability tests to be used?", choices= c("F-test on Regr."="1",
"EQ-test on lower asymptote difference"= "2",
"EQ-test on ratio of lower asymptote"= "3","EQ-test on ratio of Hill slopes"= "4",
"EQ-test on ratio of upper asymptote"= "5", "F-test on non-linearity"="6",
"EQ-test on ratio of asymptote differences"= "7"),
selected= c("1","2","3","4","5","6","7")),
#actionLink("selectall","SelectAll"),
h5("\n\n\n Author: Franz Innerbichler, InnerAnalytics")),
),
tabPanel("4pl-Analysis",
tags$style(HTML("pre { color: black; background-color: #FFE1FF;
font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;font-size: 12px;} ")),
wellPanel(
fluidRow(
column(4,
#h5("Diagnostics only shown if EXCEL is uploaded"),
htmlOutput("PureErrW2"),
tags$head(tags$style("#PureErrW2{color: red;
font-size: 16px;
font_style: italic;}")),
tableOutput("Filesampl"),
tableOutput("relpotTestTab"),
plotOutput("relpotTestPlot", width="300px", height="150px"), # Pot CI plot
h4("Akaike Information Criterion"),
tableOutput("AIC"),
h5("First row: restricted model; 2nd row: unrestricted model"),
h5("Smaller values of AIC indicate better fit to the data"),
tableOutput("VarDiagn")
),
column(8,
plotOutput("XLplot"),
plotOutput("diagnplot"),
tableOutput("ANOVAXLS"),
DTOutput("EQtests"))
))),
tabPanel("linear Analysis",
sidebarLayout(
sidebarPanel(
width=2,
fluidRow(
column(12,
numericInput("Limits",p("limit to be >", bsButton("q4",label="", icon=icon("info"), style="primary", size="extra-small")),
bsPopover(id="q4", title="", content="The calculated limits ...")),
checkboxGroupInput("selectedSSTsLinear", "Which suitability tests to be used?",
choices= c("F-test on Regr."="1",
"F-test on non-linearity"= "2",
"F-test on R^2 A"= "3","F-test on R^2 B"= "4",
"F-test on slope A"= "5", "F-test on slope B"="6",
"F-test on non-parallelism"= "7", "F-test on preparation"="8"),
selected= c("1","2","3","4","5","6","7","8")),
)
)),
mainPanel(
tabsetPanel(id="tabs",
tabPanel("linear PLA",
column(12,
htmlOutput("PureErrW3"),
tags$head(tags$style("#PureErrW3{color: red;
font-size: 16px;
font_style: italic;}")),
plotOutput("plotLin"),
"Delta method is used for potency CIs",
DT::dataTableOutput("pottab"),
h4("Unrestricted linear model (SSSI):"),
tableOutput("SummaryModABu"),
h4("Restricted linear model (CSSI):"),
tableOutput("SummaryModAB"),
h3("Tests for linear PLA):"),
box(title="Suitability tests", status="primary",solidHeader = T, width=12,
DTOutput("TESTSlin")),
h5("The estimate is the p-value of the test"),
h5("F-tests on regression, significance of slopes, and preparation need to have a p-value <0.05 to pass"),
h5("All other tests pass if p-value > 0.05"),
"SST CI for difference of slopes:",
tableOutput("SlopeDiffCI"),
h3("ANOVA for parallel line assay"),
DTOutput("ANOVAlin"))),
tabPanel("Report",
h4("Settings for report")
))
)
)
),
tabPanel("parameter estimates",
column(3,style = "background: #4FCBD922",
br(),
h4("Regression results restricted"),
tableOutput("coeffs_r"),
"Bend points restricted",
tableOutput("bends_r2")),
column(3,style = "background: #B5C74022",
br(),
h4("Regression results unrestricted"),
tableOutput("coeffs_unr")),
column(3,style = "background: #F9545422",
h4("Regression results (ln-transformed)"),
tableOutput("logcoeffs_r"),
tableOutput("bends_unr2"),
tableOutput("logcoeffs_unr"))
),
tabPanel("Report",
h4("Settings for report"),
downloadButton("downloadXLReport", label="Download PDF report", class="butt"),
tags$style(type="text/css","#downloadXLReport {background-color: orange; color: black;font-family: COurier New}"),
)
)
)
)
})
output$fourPL <- renderUI({
navbarPage(title="4PL",
tabPanel("Analysis and Plots",
#sidebarLayout(
# sidebarPanel(
# width=4,
# fluidRow(
# )
# ),
mainPanel(width=12,
tabsetPanel(id="tabs",
tabPanel("Settings",
h4("Settings of 4PL regression"),
div(checkboxInput("PureErr4pl", "Should pure error be used for calculation of CIs?", FALSE),
style = "font-size: 24px !important;color: #C2173F"),
h4("User help"),
h5("If new dilutions are entered, please adjust EC50 to avoid calculation errors"),
# numericInput("Limits",p("limit to be >", bsButton("q4",label="", icon=icon("info"), style="primary", size="extra-small")),
# bsPopover(id="q4", title="", content="The calculated limits ...")),
#h5("Diagnostics only shown if EXCEL is uploaded"),
column(2,style = "background: #7FAEFF",
#actionButton("StartCalc", "Click, when calculations to be started"),
h4("curve settings"),
numericInput("lowAsymptREF", "lower asymptote REF",10,step=1,min=0),
numericInput("lowAsymptTEST", "lower asymptote TEST",10,step=1,min=0),
numericInput("uppAsymptREF", "upper asymptote REF",110,step=1,min=0),
numericInput("uppAsymptTEST", "upper asymptote TEST",110,step=1,min=0)
),
column(2,style = "background: #7FAEFF",
numericInput("slopeREF", "slope REF",1,step=0.1,min=-10),
numericInput("slopeTEST", "slope TEST",1,step=0.1,min=-10),
numericInput("EC50", "EC50 REF",-3.5),
numericInput("potDiff", "potency difference",0)
),
column(2,style = "background: #627ADD",
h4("dilutions"),
numericInput("CONC1", "highest concentration",0.3, min=-3.5),
numericInput("CONC2", "2nd concentration",0.15),
numericInput("CONC3", "3rd concentration",0.075),
numericInput("CONC4", "4th concentration",0.0375),
numericInput("CONC5", "5th concentration",0.01875),
numericInput("CONC6", "6th concentration",0.00938)
),
column(2,style = "background: #627ADD",
numericInput("CONC7", "7th concentration",0.00469),
numericInput("CONC8", "8thd concentration",0.00235),
numericInput("CONC9", "9thd concentration",value=NA),
numericInput("CONC10", "10th concentration",value=NA),
numericInput("CONC11", "11th concentration",value=NA),
numericInput("CONC12", "lowest concentration",NA)
),
column(2,style = "background: #4FCBD9",
h4("geometric dilution scheme"),
numericInput("ConcStart", "starting concentration",value=NA, min=0),
numericInput("dilutionFac", "dilution factor",value=NA, min=0, max=10),
numericInput("NoDil", "no. of dilutions",value=NA, min=8),
numericInput("NoDilSer", "no. of dil. series",value=NA),
verbatimTextOutput("dilutions")
),
column(2,
h3("Settings"),
helpText("Vary the slider to see the effect of special cause"),
sliderInput("sdfac","Variability as % of lower to upper asymptote", max=10, value=3, min=0.1, step=0.1),
checkboxInput("heterosked","heteroskedastic noise", FALSE),
sliderInput("potencydiff","potency of test (%)", max=200, min=50, value=100, step=1),
sliderInput("outlL","outlier in lower asymptote", min=0, max=1.5, value=0, step=0.1),
sliderInput("outlM","outlier in mid part", min=0, max=1.5,value=0, step=0.1),
sliderInput("outlU","outlier in upper asymptote", min=0, max=1.5,value=0, step=0.1)
),
h4("log-dilutions from settings above"),
verbatimTextOutput("logdil")
#)
),
#### 4pl fits ----
tabPanel("4pl-fit",
tags$head(tags$style("#PureErrW2{color: red;
font-size: 10px;
font_style: italic;}")),
wellPanel(
fluidRow(
column(10,
tags$style(span(htmlOutput("PureErrW3"), style="color: red")),
htmlOutput("PureErrW3"),
plotOutput("plot", width = "80%"),
DT::dataTableOutput("pottab4pl"),
"Footnote: test performed on relative CIs.",
DTOutput("EQtests4pl"), # SSTs
h5("*...The estimate for F-test on regression and on non-linearity is the p-value"),
h5("F-test on regression passes if F-value > F-crit and thus p < 0.05"),
h5("F-test on non-linearity passes if F-value < F-crit and thus p > 0.05"),
h5("Test results outcome: 0 ... test passed (for EQ tests: CI within limits);
1 ... test failed (for EQ tests CI not within limits);
-1 ... calculations unbound/denominator too close to 0"),
#plotOutput("CIplot, height=50%")
),
column(8,
"4 PL ANOVA unrestricted",
box(title = "ANOVA unrestricted", status="warning",solidHeader = T, width=12, "",
DT::dataTableOutput("ANOVA")),
h5("Please note that for the CIs of rel. potency the RSS of the restricted model is used"),
h5("RSS ... 'Residual error' in the SumSquares column"),
h5("MSE ... 'Residual error' in the MeanSquaress column"),
h5("SSE ... 'Pure error' in the SumSquares column"),
h5("RMSE ... Square root of the 'Residual Error' in the MeanSquares column"),
verbatimTextOutput("RMSE")
),
column(8,
box(title = "Simulated data per log-concentration", status="warning",solidHeader = T, width=12, "incl. mean, sd and CV%",
DT::dataTableOutput("Conctab")))
))
),
tabPanel("ln-transformed y",
h4("ln-transformed y-axis plots"),
plotOutput("plot4plTrans", width = "80%"),
DT::dataTableOutput("pottab4plTrans"),
),
tabPanel("Report",
h4("Settings for report"),
downloadButton("downloadXLReport", label="Download PDF report", class="butt"),
tags$style(type="text/css","#downloadXLReport {background-color: orange; color: black;font-family: COurier New}"),
)
)
)
#)
)
)
})
output$pla <- renderUI({
navbarPage(title="pla",
tabPanel("Analysis and Plots",
)
)
})
output$wizard <- renderUI({
navbarPage(title="Dilution setting",
tabPanel("Plots",
sidebarLayout(
sidebarPanel(
width=3,
fluidRow(
column(6,
numericInput("Limits",p("limit to be >", bsButton("q4",label="", icon=icon("info"), style="primary", size="extra-small")),
bsPopover(id="q4", title="", content="The calculated limits ...")))
)),
mainPanel(
tabsetPanel(id="tabs",
tabPanel("4pl",
box(title="ANOVA table", status="primary",solidHeader = T, width=12,
tableOutput("Anovatab")),
column(4,
h3("Confidence intervals"),
tableOutput("CIs"),
"The confidence intrval table is interaactive for changes in: variability slider (%SD), potency of test-slider,
and 'Adjust the dilutions'-slider",
tableOutput("optimalDils"),
selectInput(inputId="scenario", label= "Select an 'optimal' scenario:", choices = c("scenario 2","scenario 3","scenario 6","steep slope"))),
column(5,
plotOutput("plotfordilutions"),
h4("in grey: most extreme bend point lines of theoretical samples with 50% and 200% potency"),
sliderInput("dilslider", "Adjust the dilutions(+-change in %)", min = -100,max=100, value=0, step=1, round=0),
checkboxInput("fixupper","Fix highest concentration (if unticked, the center is fixed)",FALSE),
h5("Dilution factors"),
tableOutput("adjlogdil"),
"Short guidance: wider dilution ranges increase the CIs of rel. potency, and decrease the CIs of upper and lower asymptote ratios, as well as Hill's slope ratios", br(),
"Narrower dilution ranges decrease the CIs of rel. potency, and increase the CIs of upper and lower asymptote ratios, ands Hill's slope ratios",),
column(3,
h3("Bend points"),
tableOutput("bps"),
tableOutput("extremebps"),
h4("Explanation of the plots")
)),
tabPanel("Report",
h4("Settings for report")
))
)
)))
})
v <- reactiveValues(num_dose=0, next.dose.t=0)
sigmoid <- reactive({
sig <- c(input$lowAsymptREF, input$lowAsymptTEST,input$uppAsymptREF,input$uppAsymptREF,
input$slopeREF,input$slopeTEST,input$EC50,input$potDiff)
sig
})
CONC <- reactive({
Konz_ <- c(input$CONC1,input$CONC2,input$CONC3,input$CONC4,
input$CONC5,input$CONC6,input$CONC7,input$CONC8,
input$CONC9,input$CONC10,input$CONC11,input$CONC12)
if (any(na.omit(Konz_)==0)) Konz_[Konz_ ==0] <- 0.0000001
Konz <- na.omit(Konz_)
})
Dils <- reactive({
Dilutions <- c(input$ConcStart,input$dilutionFac,input$NoDil,input$NoDilSer)
})
#### input EXCEL file ----
observe({
if (!is.null(input$iFile)) {
inFile <- input$iFile
ext <- tools::file_ext(inFile$name)
file.rename(inFile$datapath, paste(inFile$datapath, ".xlsx",sep=""))
t.filelocation <- gsub('\\\\','/',paste(inFile$datapath, ext,sep="."))
sheets <- openxlsx::getSheetNames(t.filelocation)
dat <- lapply(sheets, openxlsx::read.xlsx, xlsxFile = t.filelocation)
names(dat) <- sheets
Dat$wb <- dat
names(Dat$wb) <- sheets
Dat$sheets <- sheets
Dat$FileName <- input$iFile[["name"]]
}
})
output$sheetName <- renderUI({
if (!is.null(Dat$wb)) {
#browser()
cnSheets <- Dat$sheets
cnSheets2 <- c("please choose", cnSheets)
selectInput(inputId = "sheet", label="Select a sheet:",choices = cnSheets)
}
})
observeEvent(input$sign_out, {
unlink(input$iFile$datapath)
reset(id = "") # from shinyjs package
})
#### process XLSX file ----
observe({
if (!is.null(input$iFile)) {
if (!is.null(input$sheet)) {
if (input$sheet != "please choose") {
#browser()
XLdat <- Dat$wb[input$sheet][[1]]
if (is.null(XLdat)) XLdat <- Dat$wb[Dat$sheets[1]][[1]]
cn <- colnames(XLdat)
logI <- grep("log", cn)
logDoseI <- grep("log_dose", cn)
if (length(logI)>0 & length(logDoseI)==0) {
XLdat$log_dose <- XLdat[,logI]
XLdat2 <- XLdat[,-logI]
CORro <- cor(XLdat$log_dose, XLdat[,3])
} else if (length(logI)==0 & length(logDoseI)==0) {
Ind <- grep("dilu|dose|Dose|Conc|conc",cn)
XLdat$log_dose <- log(XLdat[,Ind])
CORro <- cor(XLdat[,Ind], XLdat[,3])
XLdat2 <- XLdat[,-Ind]
} else if (length(logI)>0 & length(logDoseI)>0) {
XLdat2 <- XLdat
CORro <- cor(XLdat[,logI], XLdat[,3])
}
Dat$EXCEL <- XLdat2
PureErrFlag <- input$PureErr
warning_text2 <- reactive({
ifelse(PureErrFlag, 'Pure Error is selected', '')
})
output$PureErrW2 <- renderText(warning_text2())
noDilSeries <-(ncol(XLdat2)-1)/2
noDils <- nrow(XLdat2)
Dat$noDilSeriesXL <- noDilSeries
all_l <- melt(data.frame(XLdat2), id.vars="log_dose",variable.name = "replname", value.name = "readout")
isRef <- rep(c(1,0),1,each=nrow(XLdat2)*noDilSeries)
isSample <- rep(c(0,1),1,each=nrow(XLdat2)*noDilSeries)
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
# all_l$readout[all_l$readout < 0] <- 0.01
REP$all_l <- all_l
#### XLSX eval ----
if (CORro<0) SLOPE <- -1 else SLOPE <- 1
ec50est <- (max(all_l$log_dose)+min(all_l$log_dose))/2
startlist <- list(a=min(all_l$readout), b=SLOPE, d=max(all_l$readout), cs=ec50est,r=0)
tryCatch({
mr <- gsl_nls(fn = readout ~ a+(d-a)/(1+exp(b*(cs-r*isSample-log_dose))),
data=all_l,
start=startlist,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
startlistmu <- list(as=min(all_l$readout), bs=SLOPE, ds=max(all_l$readout), cs=ec50est,
at=min(all_l$readout), bt=SLOPE, dt=max(all_l$readout), r=0)
tryCatch({
mu <- gsl_nls(fn = readout ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmu,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
err$message
})
Smr <- summary(mr)
Smu <- summary(mu)
coeffsMR <- Smr$coefficients[,1]
coeffsMU <- Smu$coefficients[,1]
Dat$coeffsMRes <- coeffsMR
Dat$coeffsMUnr <- coeffsMU
names(coeffsMU) <- c("lowAsym REF", "slope REF","upperAsym REF","EC50 REF","lowAsym TEST","slope TEST","upperAsym TEST","r")
if (!PureErrFlag) {
pot_est <- exp(confintd(mr, "r", method="asymptotic"))
potU_est <- exp(confintd(mu, "r", method="asymptotic"))
colnames(pot_est) <- c("estimate","lowerCI","upperCI")
colnames(potU_est) <- c("estimate","lowerCI","upperCI")
} else {
FitAnova <- anova(lm(readout ~ factor(log_dose)*isSample, all_l))
meanPureErr <- FitAnova[4,3]
DFsPure <- FitAnova[4,1]
VCOV <- vcov(mr)
V_V <- VCOV/Smr$sigma^2
VCOVpure <- V_V*meanPureErr
SEsPure <- sqrt(diag(V_V)*meanPureErr)
pot_est <- data.frame(estimate=exp(coeffsMR[5]), lowerCI = exp(coeffsMR[5]-qt(0.975,DFsPure)*SEsPure[5]),
upperCI = exp(coeffsMR[5]+qt(0.975,DFsPure)*SEsPure[5]))
VCOVu <- vcov(mu)
V_Vu <- VCOVu/Smu$sigma^2
#VCOVpure <- V_Vu*meanPureErr
SEsPureU <- sqrt(diag(V_Vu)*meanPureErr)
potU_est <- data.frame(estimate=exp(coeffsMU[7]), lowerCI = exp(coeffsMU[7]-qt(0.975,DFsPure)*SEsPureU[7]),
upperCI = exp(coeffsMU[7]+qt(0.975,DFsPure)*SEsPureU[7]))
}
pot <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(1,1,1,isSample))
potU <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(isSample, isSample,isSample,isSample))
SR <- summary(pot)
SU <- summary(potU)
coeffs_UN <- potU$coefficients
coeffs_UN[1] <- ifelse(xor(CORro>0, coeffs_UN[1]>0), -coeffs_UN[1],coeffs_UN[1])
coeffs_UN[2] <- ifelse(xor(CORro>0, coeffs_UN[2]>0), -coeffs_UN[2],coeffs_UN[2])
coeffs_UN[7:8] <- log(coeffs_UN[7:8])
POTU <- EDcomp(potU, percVec = c(50,50), interval="delta",display=F)
Dat$potDiffXL <- POTU[1]*100
RMSE_unr_diagn <- sqrt(SU$resVar)
RMSE_res_diagn <- sqrt(SR$resVar)
up_lowDiffDiagn <- SU$coefficients[5,1] - SU$coefficients[3,1]
ProzSD_diagn <- RMSE_unr_diagn*100/up_lowDiffDiagn
Dat$ProzSD_XL <- ProzSD_diagn
observe({
pot_est3 <- data.frame(pot_est*100)
MaxPl <- max(input$upperPot, pot_est3$upperCI)
MinPl <- min(input$lowerPot, pot_est3$lowerCI)
MaxPl_ <- MaxPl*1.2
MinPl_ <- MinPl*0.8
#browser()
p_relCI <- ggplot(data=pot_est3, aes(xmin=lowerCI, xmax=upperCI, y=1)) +
geom_linerange(size=4, col="darkseagreen",alpha=0.5) +
geom_point(aes(x=estimate, y=1), col="grey15", shape=13, size=10) +
geom_vline(xintercept = c(input$lowerPot, input$upperPot), col="indianred") +
annotate("text", x=input$lowerPot-13, y=1.040, label=paste("lower EAC:", input$lowerPot), col="indianred") +
annotate("text", x=input$upperPot+13, y=1.040, label=paste("upper EAC:", input$upperPot), col="indianred") +
annotate("text", x=pot_est3$lowerCI-10, y=1.020, label=paste("lower CL:", round(pot_est3$lowerCI,1)), col="darkgreen") +
annotate("text", x=pot_est3$upperCI+10, y=1.020, label=paste("upper CL:", round(pot_est3$upperCI,1)), col="darkgreen") +
annotate("text", x=pot_est3$estimate, y=0.98, label=paste("rel. potency:", round(pot_est3$estimate,1)), col="black") +
ylim(c(0.95, 1.05)) +
xlim(c(MinPl_,MaxPl_)) +
xlab("relative potency + confidence interval") +
theme_bw() +
theme(axis.title.y=element_blank(),
axis.text.y=element_blank(),
axis.ticks.y=element_blank())
output$relpotTestPlot <- renderPlot({
p_relCI
})
REP$relpotTestPlot <- p_relCI
output$relpotTestTab <- renderTable({ pot_est3 })
})
SStreat <- round(sum((potU$predres[,1] - mean(all_l$readout))^2),5)
SStreat_df <- length(unique(all_l$log_dose))-1
SSregr <- round(sum((predict(pot)-mean(all_l$readout))^2),5)
## Non-parallel
SSnonparallel <- round(sum(resid(pot)^2) - sum(resid(potU)^2),5)
## Preparation
SSprep <- round(sum((predict(lm(readout ~ isSample, all_l)) - mean(all_l$readout))^2),5)
## Resid Err
RSS <- round(sum(potU$predres[,2]^2),5)
RSS_df <- nrow(all_l)-SStreat_df-1
FitAnova <- anova(lm(readout ~ factor(Conc)*isSample, all_l))
# PureErr
SSE <- FitAnova[4,3]
SSE_df <- FitAnova[4,1]
# Non-Linearity
SSnonlin <- round(sum((predict(lm(readout ~ factor(Conc)*isSample, all_l)) - predict(potU))^2),4)
LoF_df <- FitAnova[1,1]+FitAnova[2,1]
## Total
SStot <- round(sum((all_l$readout -mean(all_l$readout))^2),5)
MSE <- RSS/RSS_df
noConc <- length(unique(all_l$Conc))
AnovaDFs <- c(noConc-1, 1,3,noConc-4-1, nrow(all_l)-noConc, noConc, nrow(all_l)-noConc-noConc, nrow(all_l)-1)
p_SStreat <- round(pf((SStreat/AnovaDFs[1])/MSE, AnovaDFs[1],RSS_df, lower.tail = F),3)
p_SSprep <- round(pf((SSprep/AnovaDFs[2])/MSE, AnovaDFs[2],RSS_df, lower.tail = F),3)
p_SSregr <- round(pf((SSregr/AnovaDFs[3])/MSE, AnovaDFs[3],RSS_df, lower.tail = F),3)
p_SSnonp <- round(pf((SSnonparallel/AnovaDFs[4])/MSE, AnovaDFs[3],RSS_df, lower.tail = F),3)
p_SSLoF <- round(pf((SSnonlin/LoF_df)/(SSE/SSE_df), LoF_df,SSE_df, lower.tail = F),5)
ANOVAtab2 <- data.frame(Source = c("Treatment","Preparation","Regression",
"Non-Parallelism","Residual Error","Non-linearity",
"Pure Error","Total"),
DF = round(AnovaDFs,0),
SumSquares = c(SStreat, SSprep,SSregr, SSnonparallel,
RSS, SSnonlin,SSE, SStot),
MeanSquares = c(round(SStreat/AnovaDFs[1],3), SSprep, round(SStreat/AnovaDFs[3],3),round(SSnonparallel/AnovaDFs[4],3),
round(MSE,5), round(SSnonlin/LoF_df,5), round(SSE/SSE_df,5),""),
"F-value" = c(round((SStreat/AnovaDFs[1])/MSE,5), round((SSprep/AnovaDFs[2])/MSE,5),
round((SSregr/AnovaDFs[3])/MSE,5),round((SSnonparallel/AnovaDFs[4])/MSE,5),
"",round((SSnonlin/LoF_df)/(SSE/SSE_df),5),"",""),
"p_value" = c(round(p_SStreat,3), p_SSprep, round(p_SSregr,3), p_SSnonp,"",p_SSLoF,"","")
)
output$ANOVAXLS <- renderTable({ ANOVAtab2 })
REP$ANOVAXLS <- ANOVAtab2
#browser()
startlistlog <- list(a=min(log(all_l$readout)), b=SLOPE, d=max(log(all_l$readout)), cs=ec50est,r=0)
tryCatch({
mrlog <- gsl_nls(fn = log(readout) ~ a+(d-a)/(1+exp(b*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistlog,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
print("Error in mrlog gsl_nls")
})
startlistmulog <- list(as=min(log(all_l$readout)), bs=SLOPE, ds=max(log(all_l$readout)), cs=ec50est,
at=min(log(all_l$readout)), bt=SLOPE, dt=max(log(all_l$readout)), r=0)
tryCatch({
mulog <- gsl_nls(fn = log(readout) ~ as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(cs-r*isSample-log_dose))),
data=all_l,
start=startlistmulog,
control=gsl_nls_control(xtol=1e-6,ftol=1e-6, gtol=1e-6))
},
error = function(err) {
print("Error in murlog gsl_nls")
})
logpot <- drm(log(readout) ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(1,1,1,isSample))
logpotU <- drm(log(readout) ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(isSample, isSample,isSample,isSample))
Smrlog <- summary(mrlog)$coefficients
Smulog <- summary(mulog)$coefficients
SUlog <- summary(logpotU)
SRlog <- summary(logpot)
RMSE_unrlog_diagn <- sqrt(SUlog$resVar)
RMSE_reslog_diagn <- sqrt(SRlog$resVar)
up_lowDifflogDiagn <- SUlog$coefficients[5, 1] - SUlog$coefficients[3, 1]
ProzSDlog_diagn <- RMSE_unrlog_diagn * 100 / up_lowDifflogDiagn
#### Diagnostic RMSE table ####
DiagnTable <- data.frame(parameter = c("RMSE unrestricted", "RMSE_restr.", "Diff_upper-lowerAsymp", "%SD (unrestricted)",
"RMSE log_unrestricted", "RMSE log_restr", "diff_up-lowAsymp_log", "%SD (log unrestricted)"),
result = c(round(RMSE_unr_diagn, 4), round(RMSE_res_diagn, 4),
round(up_lowDiffDiagn, 4), round(ProzSD_diagn, 4),
round(RMSE_unrlog_diagn, 4), round(RMSE_reslog_diagn, 4),
round(up_lowDifflogDiagn, 4), round(ProzSDlog_diagn, 4)))
Dat$DiagnTable <- DiagnTable
REP$DiagnTable <- DiagnTable
logpotest <- exp(confintd(mrlog, "r", method = "asymptotic")) # compParm(logpot, "c")
logpotuest <- exp(confintd(mulog, "r", method = "asymptotic")) # compParm(logpotu, "c")
# Berechnung der Konfidenzintervalle (CI)
# logpotCI <- c(exp(Smrlog[5,1] - qt(0.975, nrow(all_1)-5) * Smrlog[5,2]), exp(Smrlog[5,1]), exp(Smrlog[5,1] + qt(0.975, nrow(all_1)-5) * Smrlog[5,2]))
colnames(logpotest) <- c("estimate", "lowerCI", "upperCI")
colnames(logpotuest) <- c("estimate", "lowerCI", "upperCI")
#browser()
cnXL <- colnames(XLdat2)
Filesample <- data.frame(Test = c("File name", "samples"), Test2=c(Dat$FileName, paste(cnXL[1], " vs ", cnXL[4])))
colnames(Filesample) <- c("", "")
output$Filesampl <- renderTable({ Filesample }, rownames = F)
UnRPLAausw <- data.frame(Information = c("model", "lower asymptote Ref", "Hill's slope Ref", "upper asymptote Ref","EC50 Ref",
"lower asymptote Test", "Hill's slope Test",
"upper asymptote Test","EC50 Difference",
"relative potency", "lower CI", "upper CI"),
Results = unlist(c("UNRESTRICTED", round(coeffsMU, 3), round(potU_est*100, 3)))) # von psl_nls
# "log relative potency", "log lower CI", "log upper CI", round(logpotest, 3), round(compParm(potu, "c", display = F), 3)
output$coeffs_unr <- renderTable({
UnRPLAausw
})
#browser()
UnRPLAausw2 <- data.frame(Dat$bendpointsTRANS)
if (length(UnRPLAausw2) > 0) {
colnames(UnRPLAausw2) <- c("bendpoints log")
UnrBendLog <- data.frame(Bendpoint = c("REF_lower","REF_upper",
"TEST_lower","REF_lower"),
bendpoints_logscale = UnRPLAausw2)
output$bends_unr2 <- renderTable({
UnrBendLog
})
}
REP$UnRPLAausw <- UnRPLAausw
REP$UnRPLAausw2 <- UnRPLAausw2
# browser()
coeffs_R <- coeffsMR # pot$coefficients
coeffs_R[5] <- coeffs_R[4] - coeffs_R[5]
names(coeffs_R) <- c("lower A", "slope", "upper A", "EC50 REF", "EC50 TEST")
# coeffs_R[4] <- log(coeffs_R[4])
# coeffs_R[5] <- log(coeffs_R[5])
# --- Ergebnistabelle: RESTRICTED (Eingeschränktes Modell) ---
PLAAusw <- data.frame(
Information = c("model", "lower asymptote", "Hill's slope", "upper asymptote","EC50 Ref",
"EC50 Test", "relative potency",
"lower CI", "upper CI"),
Results = unlist(c("RESTRICTED", round(coeffs_R, 3),
round(pot_est[1, ] * 100, 3)))) # von gs1_nls
output$coeffs_r <- renderTable({ PLAAusw })
PLAAusw2 <- data.frame(Dat$bendpoints)
output$bends_r2 <- renderTable({ PLAAusw2 }, digits = 3, rownames = T)
REP$PLAausw <- PLAAusw
REP$PLBend <- PLAAusw2
# --- Koeffizienten-Extraktion ---
logcoeffs_R <- Smrlog[, 1] # logpot$coefficients
names(logcoeffs_R) <- c("lower A", "Hill's slope", "upper A", "EC50 REF","EC50 DIFF")
# --- Ergebnistabelle: LOG RESTRICTED ---
LogPLAAusw <- data.frame(
Information = c("model", "lower asymptote", "Hill's slope", "upper asymptote","EC50 Ref",
"EC50 difference", "log relative potency",
"log lower CI", "log upper CI"),
Results = unlist(c("LOG RESTRICTED", round(logcoeffs_R, 3),
round(logpotest * 100, 3)))) # von gs1_nls
output$logcoeffs_r <- renderTable({ LogPLAAusw })
REP$LogPLAausw <- LogPLAAusw
logcoeffs_UNR <- Smulog[,1]
names(logcoeffs_UNR) <- c("lower asymptote Ref", "Hill's slope Ref", "upper asymptote Ref","EC50 Ref",
"lower asymptote Test", "Hill's slope Test", "upper asymptote Test","EC50 Diff"
)
# --- Ergebnistabelle: LOG UNRESTRICTED ---
LogUnrPLAAusw <- data.frame(
Information = c("model", "lower asymptote Ref", "Hill's slope Ref", "upper asymptote Ref","EC50 Ref",
"lower asymptote Test", "Hill's slope Test", "upper asymptote Test","EC50 Diff" ,
"relative potency", "lower CI", "upper CI"),
Results = unlist(c("LOG UNRESTRICTED", round(logcoeffs_UNR, 3),
round(logpotest * 100, 3)))) # von gs1_nls
output$logcoeffs_unr <- renderTable({
LogUnrPLAAusw
})
REP$LogUnrPLAausw <- LogUnrPLAAusw
#browser()
Dat$coeffs_UN <- coeffs_UN
if (exists("Ind")) {
Dat$dilution <- XLdat[,Ind]
} else Dat$dilution <- exp(XLdat[,logI])
# --- Plot-Ausgabe ---
output$XLplot <- renderPlot({
plot_f(XLdat2, sigmoid = NULL, det_sig = coeffs_UN, TransFlag=F)
})
REP$XLdat2 <- XLdat2
# --- Diagnose-Plots (Residualanalyse) ---
output$diagnplot <- renderPlot({
op <- par(mfrow = c(2, 2), mar = c(3.2, 3.2, 2, .5), mgp = c(2, .7, 0))
# 1. Residuals vs Fitted
plot(residuals(pot) ~ fitted(pot), main = "Residuals restricted")
abline(h = 0)
qqnorm(residuals(pot))
qqline(residuals(pot))
plot(residuals(potU) ~ fitted(potU), main = "Residuals unrestricted")
abline(h = 0)
qqnorm(residuals(potU))
qqline(residuals(potU))
par(op) # Parameter zurücksetzen
})
output$AIC <- renderTable({
AIC <- AIC(pot, potU)
})
output$VarDiagn <- renderTable({
DiagnTable
}, digits=4)
output$relpotplot <- renderPlot({
relpot(potU, intervall="fieller", bty="l",
main="Quality of rel. potency over response")
})
} # !please choose
} # input$sheet
} # input$iFile
})
#### make geomDils reactive ----
observe({
#browser()
if (is.null(input$ConcStart)) return(NULL)
if (!is.na(input$ConcStart) & !is.na(input$dilutionFac) &!is.na(input$NoDil) &!is.na(input$NoDilSer)) {
upR <- input$ConcStart
noDil <- input$NoDil
noDilSer <- input$NoDilSer
Div <- input$dilutionFac
res <- c()
N_ <- 1
Conc <- c(upR, divFUN(upR,Div,N=N_,res,noDil))
Dat$MetaConc <- Conc
}
})
#### updateSlider on XLSX ----
observe({
if (!is.null(Dat$potDiffXL)) {
updateSliderInput(session, "potencydiff",
value=round(as.numeric(Dat$potDiffXL[[1]]),5))
}
})
observeEvent(input$potencydiff, {
if (!is.null(Dat$potDiffXL)) {
updateSliderInput(session, "potencydiff",
value=round(as.numeric(input$potencydiff),5))
}
})
observe({
if (!is.null(Dat$ProzSD_XL)) {
updateSliderInput(session, "sdfacf",
value=round(as.numeric(Dat$ProzSD_XL[[1]]),5))
}
})
observeEvent(input$sdfac, {
if (!is.null(Dat$ProzSD_XL)) {
updateSliderInput(session, "sdfac",
value=round(as.numeric(Dat$ProzSD_XL[[1]]),5))
}
})
#### updaterNumeric Input ----
observe({
if(!is.null(Dat$coeffs_UN)) {
updateNumericInput(session, "lowAsymptREF",
value=round(as.numeric(Dat$coeffs_UN[3]),5), min=0)
updateNumericInput(session, "lowAsymptTEST",
value=round(as.numeric(Dat$coeffs_UN[4]),5), min=0)
updateNumericInput(session, "uppAsymptREF",
value=round(as.numeric(Dat$coeffs_UN[5]),5), min=0)
updateNumericInput(session, "uppAsymptTEST",
value=round(as.numeric(Dat$coeffs_UN[6]),5), min=0)
updateNumericInput(session, "slopeREF",
value=round(as.numeric(Dat$coeffs_UN[1]),5))
updateNumericInput(session, "slopeTEST",
value=round(as.numeric(Dat$coeffs_UN[2]),5))
updateNumericInput(session, "EC50",
value=round(as.numeric(Dat$coeffs_UN[7]),5))
updateNumericInput(session, "potDiff",
value=round(as.numeric(Dat$coeffs_UN[7])- as.numeric(Dat$coeffs_UN[8]),5))
}
})
observe({
if(!is.null(Dat$dilution)) {
updateNumericInput(session, "CONC1",
value=as.numeric(Dat$dilution[1]))
updateNumericInput(session, "CONC2",
value=as.numeric(Dat$dilution[2]))
updateNumericInput(session, "CONC3",
value=as.numeric(Dat$dilution[3]))
updateNumericInput(session, "CONC4",
value=as.numeric(Dat$dilution[4]))
updateNumericInput(session, "CONC5",
value=as.numeric(Dat$dilution[5]))
updateNumericInput(session, "CONC6",
value=as.numeric(Dat$dilution[6]))
updateNumericInput(session, "CONC7",
value=as.numeric(Dat$dilution[7]))
updateNumericInput(session, "CONC8",
value=as.numeric(Dat$dilution[8]))
updateNumericInput(session, "CONC9",
value=as.numeric(Dat$dilution[9]))
updateNumericInput(session, "CONC10",
value=as.numeric(Dat$dilution[10]))
updateNumericInput(session, "CONC11",
value=as.numeric(Dat$dilution[11]))
updateNumericInput(session, "CONC12",
value=as.numeric(Dat$dilution[12]))
}
})
observe({
if(!is.null(Dat$MetaConc)) {
updateNumericInput(session, "CONC1",
value=as.numeric(Dat$MetaConc[1]))
updateNumericInput(session, "CONC2",
value=as.numeric(Dat$MetaConc[2]))
updateNumericInput(session, "CONC3",
value=as.numeric(Dat$MetaConc[3]))
updateNumericInput(session, "CONC4",
value=as.numeric(Dat$MetaConc[4]))
updateNumericInput(session, "CONC5",
value=as.numeric(Dat$MetaConc[5]))
updateNumericInput(session, "CONC6",
value=as.numeric(Dat$MetaConc[6]))
updateNumericInput(session, "CONC7",
value=as.numeric(Dat$MetaConc[7]))
updateNumericInput(session, "CONC8",
value=as.numeric(Dat$MetaConc[8]))
updateNumericInput(session, "CONC9",
value=as.numeric(Dat$MetaConc[9]))
updateNumericInput(session, "CONC10",
value=as.numeric(Dat$MetaConc[10]))
updateNumericInput(session, "CONC11",
value=as.numeric(Dat$MetaConc[11]))
updateNumericInput(session, "CONC12",
value=as.numeric(Dat$MetaConc[12]))
}
})
#### render logDilsText ----
output$logdil <- renderText({
if (!is.null(Dat$MetaConc)) {
Conc <- Dat$MetaConc
} else Conc <- CONC()
logdilu <-log(Conc)
logdilu
})
#### reactive dataset sim ----
sim <- reactive({
#browser()
if(is.null(sigmoid())) return(NULL)
sd_fac_ <- as.numeric(input$sdfac)
r_ <- log(as.numeric(input$potencydiff)/100)
as = sigmoid()[1]; bs = sigmoid()[5];cs = sigmoid()[7];ds = sigmoid()[3];at = sigmoid()[2];
bt = sigmoid()[6];r = sigmoid()[8]; ct = cs-r_; dt = sigmoid()[4];
if (!is.null(Dat$MetaConc)) Conc <- Dat$MetaConc else Conc <- CONC()
log_conc <- log(Conc)
av_test <- as + (ds-as)/(1+exp(bs*(cs - log_conc)))
av_ref <- at + (dt-at)/(1+exp(bt*(ct - log_conc)))
#browser()
if (!is.na(input$NoDilSer)) {
noDilSer <- input$NoDilSer
} else if (!is.null(Dat$noDilSeriesXL)) noDilSer <- Dat$noDilSeriesXL else noDilSer <- 3
if (!is.na(input$NoDil)) noDil <- input$NoDil else noDil <- length(log_conc)
isRef <- rep(c(1,0), 1,each=noDilSer*noDil)
isSample <- rep(c(0,1), 1,each=noDilSer*noDil)
#if (is.null(Dat$EXCEL)) {
ro_new <- Calc_DilRes(as=as,at=at,ds=ds,dt=dt,cs=cs,ct=ct,r=r_,bt=bt,bs=bs, log_conc = log_conc,
sd_fac=sd_fac_,
# auslenkU=outlierU,
# auslenkM=outlierM,
# auslenkL=outlierL,
heteroNoise = input$heterosked, noDilSeries = noDilSer, noDils = noDil)
#} else ro_new <- Dat$EXCEL
})
# })
####sim2 ----
sim2 <- reactive({
tab <- sim()
if (is.null(Dat$EXCEL)) return(tab) else return(Dat$EXCEL)
})
#### Plot 4pl ----
output$plot <- renderPlot({
#browser()
sigmoid <- sigmoid()
det_sig=NULL
plot_f(sim2(),sigmoid, det_sig, TransFlag = F)
})
#### Plot 4pl Transformed ----
output$plot4plTrans <- renderPlot({
#browser()
sigmoid <- sigmoid()
det_sig=NULL
plot_f(sim2(),sigmoid, det_sig, TransFlag = T)
})
#### Testergebnisse für 4PL ----
observe({
if (is.null(sim2())) return(NULL)
if (is.null(input$PureErr4pl)) return(NULL)
#observeEvent(input$StartCalc,{
PureErrFlag <- input$PureErr4pl
warning_text3 <- reactive({
ifelse(PureErrFlag, 'Pure error selected','')
})
#browser()
output$PureErrW3 <- renderText(warning_text3())
Limite <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot),
as.numeric(input$lEACratioAdiff), as.numeric(input$uEACratioAdiff))
Dat$limite <- Limite
#browser()
tab <- tests_FUNC(sim2(), Limite, PureErrFlag = PureErrFlag)
if (length(tab)>1) {
tab[1,6:7] <- c("-","-")
Dat$tests_FUNC <- tab
REP$testsTab <- tab
tab2 <- tab[1:7,]
dat <- datatable(tab2,options = list(
paging=TRUE,
dom="t",
rownames=FALSE
)) %>% formatStyle("test_results",
target='row',
backgroundColor = styleEqual(c(-1,0,1),
c("pink",'lightgreen','lightgrey')))
} else { dat <- datatable(data.frame(test_results = "Convergeance failed for the uploaded dataset")) }
#browser()
output$EQtests4pl <- renderDT({ dat})
}) # observe
#### Testergebnisse für XLSX ----
observe({
if (is.null(Dat$EXCEL)) return(NULL)
if (is.null(input$PureErr)) return(NULL)
#observeEvent(input$StartCalc,{
PureErrFlag <- input$PureErr
warning_text3 <- reactive({
ifelse(PureErrFlag, 'Pure error selected','')
})
output$PureErrW3 <- renderText(warning_text3())
Limite <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot),
as.numeric(input$lEACratioAdiff), as.numeric(input$uEACratioAdiff))
Dat$limite <- Limite
#browser()
SelTests <- as.numeric(input$selectedSSTs)
tab <- tests_FUNC(Dat$EXCEL, Limite, PureErrFlag = PureErrFlag)
tab[1,6:7] <- c("-","-")
Dat$tests_FUNC <- tab
REP$testsTab <- tab
tab2 <- tab[SelTests,]
dat <- datatable(tab2,options = list(
paging=TRUE,
dom="t",
rownames=FALSE
)) %>% formatStyle("test_results",
target='row',
backgroundColor = styleEqual(c(-1,0,1),
c("pink",'lightgreen','lightgrey')))
output$EQtests <- renderDT({ dat })
}) # observe
####plot CIs ----
observe({
tab <- Dat$tests_FUNC
if (is.null(tab)) return(NULL)
tab2 <- tab[-c(1,2,3,6),]
tab2[,3:7] <- apply(tab2[,3:7],2,as.numeric)
tab2[4:5,3:7] <- tab2[4:5,3:7]/100
p_CIs <- ggplot(tab2,aes(x=test,y=estimate, color=test,group=test)) +
geom_point() +
geom_errorbar(aes(ymin=lower_CI, ymax=upper_CI), width=0.4) +
geom_crossbar(aes(ymin=lower_limit, ymax=upper_limit), size=0.8) +
coord_flip() +
theme_bw() +
theme(legend.position = "none",text = element_text(size=20))
output$CIplot <- renderPlot({ p_CIs}, height=200)
REP$CIplot <- p_CIs
})
output$simdat <- DT::renderDataTable({
tab <- sim2()
if (is.character(tab)) stop(tab)
tab2 <- round(tab, 5)
colnames(tab2) <- c(paste("T", seq(1,(ncol(tab2)-1)/2)),
paste("R", seq(1,(ncol(tab2)-1)/2)), "log_conc" )
dat <- datatable(tab2, options=list(
paging=T,
pageLength=20,
dom="t"
))
})
output$Conctab <- DT::renderDataTable({
if (!is.na(Dils()[1]) & is.na(Dils()[4])) return(NULL)
tab <- sim2()
if (is.character(tab)) stop(tab)
if (!is.na(Dils()[4])) {
noDilSer <- Dils()[4]
} else if (!is.null(Dat$noDilSeriesXL)) {
noDilSer <- Dat$noDilSeriesXL
} else { noDilSer <- 3 }
Conc <- CONC()
Conctab <- perConcTab(tab, noDilSeries = noDilSer)
Dat$Conctab <- Conctab
dat <- datatable(Conctab, options=list(
paging=T,
pageLength=12,
dom="t"
)) %>% formatStyle(0,
target='row',
backgroundColor = styleEqual(c("avs","sds","cv", "avs_test","sds_test","cv_test"),
c('lightgrey','lightgreen','pink','lightgrey','lightgreen','pink'))
) %>% formatRound(columns=colnames(Conctab), digits=3)
})
#### linear Plot output ----
output$plotLin <- renderPlot({
tab <- Dat$EXCEL
# tab <- sim2()
if (is.character(tab)) stop(tab)
#browser()
log_conc <- tab$log_dose
noDilSer = (ncol(tab)-1)/2
noDil <- nrow(tab)
Conctab <- perConcTab(tab, noDilSer)
# if (!is.na(Dils()[3])) noDil <- Dils()[3] else noDil = length(Conc)
#
slopeSt <- slopeTe <- matrix(NA, nrow=noDil-2,ncol=2)
for (i in 1:(noDil-2)) {
avs <- Conctab[noDilSer+1,]
threes <- data.frame(lnC=log_conc[i:(i+2)], resp=avs[i:(i+2)])
lm3St <- lm(resp ~ lnC, data=threes)
slopeSt[i,] <- lm3St$coefficients
avt <- Conctab[noDilSer*2+4,]
threet <- data.frame(lnC=log_conc[i:(i+2)], resp=avt[i:(i+2)])
lm3Te <- lm(resp ~ lnC, data=threet)
slopeTe[i,] <- lm3Te$coefficients
}
indS <- which(abs(slopeSt[,2]) == max(abs(slopeSt[,2])))
indT <- which(abs(slopeTe[,2]) == max(abs(slopeTe[,2])))
pl_ <- slopeSt[indS,1]+slopeSt[indS,2]*log_conc
pl_T <- slopeTe[indT,1]+slopeTe[indT,2]*log_conc
pl_df <- data.frame(lnC=log_conc, plotS=pl_, plotT=pl_T)
all_l <- melt(data.frame(tab), id.vars="log_dose",variable.name="replname",value.name="readout")
isRef <- rep(c(1,0), 1,each=nrow(all_l)/2)
isSample <- rep(c(0,1), 1,each=nrow(all_l)/2)
all_l2 <- cbind(all_l,isRef, isSample)
all_l2S <- all_l2[all_l2$isRef == 1,]
all_l2T <- all_l2[all_l2$isRef == 0,]
all_mS <- all_l2S[order(all_l2S$log_dose, decreasing=TRUE),]
all_mT <- all_l2T[order(all_l2T$log_dose, decreasing=TRUE),]
circleS <- all_mS[(indS*noDilSer-(noDilSer-1)):((indS+2)*noDilSer),]
circleT <- all_mT[(indT*noDilSer-(noDilSer-1)):((indT+2)*noDilSer),]
circle <- rbind(circleS,circleT)
Dat$circles <- circle
#browser()
mLin <- gsl_nls(readout ~ (intS+r)*isSample + intS*isRef + k*log_dose,
data=circle,
start=list(intS = 0, k=1,r=0),
control = gsl_nls_control(xtol=1e-10,ftol=1e-10,gtol=1e-10))
# alternativ: modAB <- lm(readout ~ log_dose+isSample, circle)
sum_mLin <- summary(mLin)
sigmoid <- Dat$coeffsMUnr
log_dose <- unique(all_l$log_dose)
seq_x <- seq(min(log_dose), max(log_dose),0.1)
SAMPLEtrue <- sigmoid[5] + (sigmoid[7]-sigmoid[5])/(1+exp(sigmoid[6]*((sigmoid[4]-sigmoid[8]-seq_x))))
REFtrue <- sigmoid[1] + (sigmoid[3]-sigmoid[1])/(1+exp(sigmoid[2]*((sigmoid[4]-seq_x))))
truePL_df <- cbind(seq_x,SAMPLEtrue, REFtrue)
p <- ggplot(all_l2,aes(x=log_dose,y=readout, color=factor(isRef))) +
geom_point() +
labs(title=paste("linear regression model", indS,indT), color="product") +
scale_colour_manual(labels = c("test","reference"), values=c("#C2173F","#4545BA")) +
ylim(min(all_l2$readout),max(all_l2$readout)) +
theme_bw()
p2 <- p + geom_line(data=pl_df,aes(x=lnC,y=plotS),color="#4545BA",
inherit.aes = F) +
geom_line(data=pl_df,aes(x=lnC,y=plotT),color="#C2173F",
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=SAMPLEtrue),color="#C2173F", linetype=2,alpha=0.4,
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=REFtrue),color="#4545BA", linetype=2,alpha=0.4,
inherit.aes = F) +
labs(title = paste("unrestricted linear regression model",indS,indT), color="product") +
theme(legend.position="none", axis.text = element_text(size=14))
p3 <- p2 + geom_point(circle, mapping=aes(x=log_dose, y=readout, shape=factor(isRef),
size=5,alpha=0.2), inherit.aes = FALSE) +
scale_shape_manual(labels=c("test","reference"), values=c(21,21))
# fit intercept for test and ref and common slope
pl_restS <- sum_mLin$coefficients[1,1] + sum_mLin$coefficients[2,1]*log_conc
pl_restT <- sum_mLin$coefficients[1,1] + sum_mLin$coefficients[3,1] + sum_mLin$coefficients[2,1]*log_conc
pl_rest <- data.frame(lnC=log_conc, plotS=pl_restS, plotT=pl_restT)
pr2 <- p + geom_line(data=pl_rest,aes(x=lnC,y=plotS),color="#4545BA",
inherit.aes = F) +
geom_line(data=pl_rest,aes(x=lnC,y=plotT),color="#C2173F",
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=SAMPLEtrue),color="#C2173F", linetype=2,alpha=0.4,
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=REFtrue),color="#4545BA", linetype=2,alpha=0.4,
inherit.aes = F) +
labs(title = paste("restricted linear regression model",indS,indT), color="product") +
theme(legend.position="none", axis.text = element_text(size=14))
pr3 <- pr2 + geom_point(circle, mapping=aes(x=log_dose, y=readout, shape=factor(isRef),
size=5,alpha=0.2), inherit.aes = FALSE) +
scale_shape_manual(labels=c("test","reference"), values=c(21,21))
grid.arrange(p3,pr3,nrow=1)
})
output$plotLin2 <- renderPlot({
tab <- sim2()
if (is.character(tab)) stop(tab)
#browser()
if (!is.na(Dils()[4])) noDilSer <- Dils()[4] else noDilSer = (ncol(tab)-1)/2
Conc <- CONC()
Conctab <- Dat$Conctab
if (!is.na(Dils()[3])) noDil <- Dils()[3] else noDil = length(Conc)
slopeSt <- slopeTe <- matrix(NA, nrow=noDil-2,ncol=2)
for (i in 1:(noDil-2)) {
avs <- Conctab[noDilSer+1,]
threes <- data.frame(lnC=log(Conc[i:(i+2)]), resp=avs[i:(i+2)])
lm3St <- lm(resp ~ lnC, data=threes)
slopeSt[i,] <- lm3St$coefficients
avt <- Conctab[noDilSer*2+4,]
threet <- data.frame(lnC=log(Conc[i:(i+2)]), resp=avt[i:(i+2)])
lm3Te <- lm(resp ~ lnC, data=threet)
slopeTe[i,] <- lm3Te$coefficients
}
indS <- which(abs(slopeSt[,2]) == max(abs(slopeSt[,2])))
indT <- which(abs(slopeTe[,2]) == max(abs(slopeTe[,2])))
pl_ <- slopeSt[indS,1]+slopeSt[indS,2]*log_conc
pl_T <- slopeTe[indT,1]+slopeTe[indT,2]*log_conc
pl_df <- data.frame(lnC=log_conc, plotS=pl_, plotT=pl_T)
all_l <- melt(data.frame(tab), id.vars="log_dose",variable.name="replname",value.name="readout")
isRef <- rep(c(1,0), 1,each=nrow(all_l)/2)
isSample <- rep(c(0,1), 1,each=nrow(all_l)/2)
all_l2 <- cbind(all_l,isRef, isSample)
all_l2S <- all_l2[all_l2$isRef == 1,]
all_l2T <- all_l2[all_l2$isRef == 0,]
all_mS <- all_l2S[order(all_l2S$log_dose, decreasing=TRUE),]
all_mT <- all_l2T[order(all_l2T$log_dose, decreasing=TRUE),]
circleS <- all_mS[(indS*noDilSer-(noDilSer-1)):((indS+2)*noDilSer),]
circleT <- all_mT[(indT*noDilSer-(noDilSer-1)):((indT+2)*noDilSer),]
circle <- rbind(circleS,circleT)
Dat$circles <- circle
sigmoid <- sigmoid()
log_dose <- unique(all_l$log_dose)
seq_x <- seq(min(log_dose), max(log_dose),0.1)
SAMPLEtrue <- sigmoid[2] + (sigmoid[4]-sigmoid[2])/(1+exp(sigmoid[6]*((sigmoid[7]-log(input$potencydiff/100)-seq_x))))
REFtrue <- sigmoid[1] + (sigmoid[3]-sigmoid[1])/(1+exp(sigmoid[5]*((sigmoid[7]-seq_x))))
truePL_df <- cbind(seq_x,SAMPLEtrue, REFtrue)
p <- ggplot(all_l2,aes(x=log_dose,y=readout, color=factor(isRef))) +
geom_point() +
labs(title=paste("linear regression model", indS,indT), color="product") +
scale_colour_manual(labels = c("test","reference"), values=c("red","blue")) +
ylim(min(all_l2$readout),max(all_l2$readout)) +
theme_bw()
p2 <- p + geom_line(data=pl_df,aes(x=lnC,y=plotS),color="blue",
inherit.aes = F) +
geom_line(data=pl_df,aes(x=lnC,y=plotT),color="red",
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=SAMPLEtrue),color="red", linetype=2,alpha=0.4,
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=REFtrue),color="blue", linetype=2,alpha=0.4,
inherit.aes = F) +
labs(title = paste("unrestricted linear regression model",indS,indT), color="product") +
theme(legend.position="none", axis.text = element_text(size=14))
p3 <- p2 + geom_point(circle, mapping=aes(x=log_dose, y=readout, shape=factor(isRef),
size=5,alpha=0.2), inherit.aes = FALSE) +
scale_shape_manual(labels=c("test","reference"), values=c(21,21))
mLin <- gsl_nls(readout ~ (intS+r)*isSample + intS*isRef + k*log_dose,
data=circle,
start=list(intS = 0, k=1,r=0),
control = gsl_nls_control(xtol=1e-10,ftol=1e-10,gtol=1e-10))
# alternativ: modAB <- lm(readout ~ log_dose+isSample, circle)
sum_mLin <- summary(mLin)
pl_restS <- sum_mLin$coefficients[1,1] + sum_mLin$coefficients[2,1]*log_conc
pl_restT <- sum_mLin$coefficients[1,1] + sum_mLin$coefficients[3,1] + sum_mLin$coefficients[2,1]*log_conc
pl_rest <- data.frame(lnC=log_conc, plotS=pl_restS, plotT=pl_restT)
pr2 <- p + geom_line(data=pl_rest,aes(x=lnC,y=plotS),color="blue",
inherit.aes = F) +
geom_line(data=pl_rest,aes(x=lnC,y=plotT),color="red",
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=SAMPLEtrue),color="red", linetype=2,alpha=0.4,
inherit.aes = F) +
geom_line(data=data.frame(truePL_df),aes(x=seq_x,y=REFtrue),color="blue", linetype=2,alpha=0.4,
inherit.aes = F) +
labs(title = paste("restricted linear regression model",indS,indT), color="product") +
theme(legend.position="none", axis.text = element_text(size=14))
pr3 <- pr2 + geom_point(circle, mapping=aes(x=log_dose, y=readout, shape=factor(isRef),
size=5,alpha=0.2), inherit.aes = FALSE) +
scale_shape_manual(labels=c("test","reference"), values=c(21,21))
grid.arrange(p3,pr3,nrow=1)
})
#### linear PLA tests ----
output$TESTSlin <- DT::renderDataTable({
tab <- sim2()
if (is.character(tab)) stop(tab)
Conc <- CONC()
Limite <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot),
as.numeric(input$lEACratioAdiff), as.numeric(input$uEACratioAdiff))
circles <- Dat$circles
PureErrFlag <- input$PureErr
warning_text <- reactive({
ifelse(PureErrFlag, 'Pure error is selected','')
})
output$PureErrW <- renderText(warning_text())
LIN <- ANOVAlintests(tab,circles,Limite,PureErrFlag=PureErrFlag)
df <- LIN[[1]]
su_modU <- LIN[[2]]
su_mod2 <- LIN[[4]]
output$SummaryModABu <- renderTable({ su_modU }, digits=5)
output$SummaryModAB <- renderTable({ su_mod2 }, digits=5)
slopeDiffCI <- t(data.frame(LIN[[3]]))
colnames(slopeDiffCI) <- c("slope difference","lower CI","upper CI")
output$SlopeDiffCI <- renderTable({ slopeDiffCI },digits=5)
#browser()
Dat$ANOVA <- df[,4:length(df)]
dat <- datatable(df[,1:3],
options=list(
paging=T, dom="t",rownames=F
)) %>% formatStyle("test_results", target="row",backgroundColor = styleEqual(c(-1,0,1),
c("pink","lightgreen","lightgrey")))
})
#### output 4PL ANOVA tests ---
output$ANOVA <- DT::renderDataTable({
sigmoid <- sigmoid()
tab <- ANOVA4plUnresfunc(sim2(),sigmoid)
dat <- datatable(tab,
options=list(
dom="t",rownames=F
)) %>% formatStyle("p_value", target="row",
backgroundColor = styleEqual(c("p_value"),
c("lightgrey")))
})
#### output RMSEs ----
output$RMSE <- renderText({
paste("RMSE (unrestricted model):", Dat$RMSE_unr, "(~ entered % upper-lower asymptote)\n",
"RMSE restricted model:", Dat$RMSE_r, "\n",
"Pure RMSE unrestricted model:", Dat$RMSE_pure, "\n",
"%SD (unr model): ", Dat$RMSE_unr*100/Dat$up_lowAs, "(calculated as: RMSE/(upper-lower Asymptote)*100\n",
"RMSE (log restr. model): ", Dat$RMSE_Rlog, "\n",
"RMSE (log unrestr. model): ", Dat$RMSE_Ulog, "\n",
"%SDlog (unr model): ", Dat$RMSE_Ulog*100/Dat$up_lowAslog )
})
output$ANOVAlin <- DT::renderDataTable({
ANOVAlin <- Dat$ANOVA
dat <- datatable(ANOVAlin,
options=list(
dom="t",rownames=F
)) %>% formatStyle("p.value", target='cell',
backgroundColor = styleEqual(c("p.value"),
c("lightgrey")))
})
### output pot tab ----
output$pottab <- DT::renderDataTable({
Lim <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot))
circles <- Dat$circles
PureErrFlag <- input$PureErr
pottab <- LinPotTab(circles,Lim,PureErrFlag = PureErrFlag)
#browser()
dat <- datatable(pottab,
options=list(
dom="t",rownames=F
)) %>% formatStyle("test_result", target='row',
backgroundColor = styleEqual(c(0,1), c("lightgrey")))
})
#### 4pl potency table ----
observe({
#browser()
if (is.null(sim2()) | is.null(Dils())) return(NULL)
ro_new <- sim2()
Dils_ <- Dils()
if (!is.na(Dils()[4])) noDilSer <- Dils()[4] else noDilSer <- 3
PureErrFl <- input$PureErr4pl
pottab4 <- pot4plFUNC(ro_new = ro_new, PureErrFlag = PureErrFl)
#browser()
Lim <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot))
pottab4_ <- data.frame(pottab4)
pottab4_$potency <- as.numeric(pottab4[,2])*100
pottab4_$`lower95%CI` <- as.numeric(pottab4[,3])*100
pottab4_$`upper95%CI` <- as.numeric(pottab4[,4])*100
pottab4_$relative_lowerCL <- round(pottab4_[,6]/pottab4_[,5]*100,3)
pottab4_$relative_upperCL <- round(pottab4_[,7]/pottab4_[,5]*100,3)
if (as.numeric(pottab4_$relative_lowerCL[1]) > Lim[[9]] & as.numeric(pottab4_$relative_upperCL[1]) < Lim[[10]] ) {
test_potCI <- 0
} else {test_potCI <- 1 }
if (as.numeric(pottab4_$relative_lowerCL[2]) > Lim[[9]] & as.numeric(pottab4_$relative_upperCL[2]) < Lim[[10]] ) {
test_potUCI <- 0
} else {test_potUCI <- 1 }
if (as.numeric(pottab4_$relative_lowerCL[3]) > Lim[[9]] & as.numeric(pottab4_$relative_upperCL[3]) < Lim[[10]] ) {
test_potCI_t <- 0
} else {test_potCI_t <- 1 }
if (as.numeric(pottab4_$relative_lowerCL[4]) > Lim[[9]] & as.numeric(pottab4_$relative_upperCL[4]) < Lim[[10]] ) {
test_potUCI_t <- 0
} else {test_potUCI_t <- 1 }
pottab4_ <- cbind(pottab4_[,-(2:4)], data.frame(tests=c(test_potCI, test_potUCI,test_potCI_t,test_potUCI_t)))
colnames(pottab4_) <- c("model","potency","lower95%CI","upper95%CI","relative_lower95%CI","relative_upper95%CI","test_result")
output$pottab4pl <- DT::renderDataTable({
dat <- datatable(pottab4_[1:2,],
options=list(
paging=T, dom="t",rownames=F
)) %>% formatStyle("test_result", target="row",backgroundColor = styleEqual(c(0,1),
c("lightgreen","pink")))
})
output$pottab4plTrans <- DT::renderDataTable({
dat <- datatable(pottab4_[3:4,],
options=list(
paging=T, dom="t",rownames=F
)) %>% formatStyle("test_result", target="row",backgroundColor = styleEqual(c(0,1),
c("lightgreen","pink")))
})
})
#### Dilutions Simulator ----
output$plotfordilutions <- renderPlot({
tab <- sim2()
#browser()
tab <- as.data.frame(tab)
dils <- tab$log_dose
min_y <- min(tab[,1:3])
max_y <- max(tab[,1:3])
if (input$fixupper) {
dils_av <- dils-max(dils)
dils_av_ <- dils_av*(input$dilslider/100+1)
dils2 <- round(dils_av_ + max(dils),4)
dilfactors <- 1/exp(dils2-lag(dils2))
} else {
if (!is.null(Dat$cfordils)) {
av <- Dat$cfordils
} else { av <- (min(dils) + max(dils))/2 }
dils_av <- dils-av
dils_avsc <- dils_av*(input$dilslider/100+1)
dils2 <- dils_avsc+av
dilfactors <- 1/exp(dils2-lag(dils2))
}
Dat$newDils <- dils2
sigmoid <- sigmoid()
#browser()
BPs <- Dat$bendpoints
EC50REF <- (BPs[2]+BPs[1])/2
Einh <- abs((BPs[2]-BPs[1])/5)
asyml <- EC50REF-2*(EC50REF-BPs[1])
asymu <- EC50REF+2*(EC50REF-BPs[1])
det_sig <- Dat$coeffs_UN
if (is.null(Dat$coeffs_UN)) {
SAMPLE50 <- sigmoid[1] + (sigmoid[3] - sigmoid[1])/(1+exp(sigmoid[5]*( (sigmoid[7]+0.693147)- dils2)))
SAMPLE200 <- sigmoid[1] + (sigmoid[3] - sigmoid[1])/(1+exp(sigmoid[5]*( (sigmoid[7]-0.693147)-dils2)))
Xbend50l <- sigmoid[7] + 0.693147-1.31696/sigmoid[5]
Xbend200l <- sigmoid[7] - 0.693147-1.31696/sigmoid[5]
Xbend50u <- sigmoid[7] + 0.693147+1.31696/sigmoid[5]
Xbend200u <- sigmoid[7] - 0.693147+1.31696/sigmoid[5]
Xbend50 <- max(Xbend50l, Xbend50u)
Xbend200 <- min(Xbend200l, Xbend200u)
dummy <- plot_f(tab,sigmoid,det_sig=NULL)
} else {
#browser()
SAMPLE50 <- det_sig[3] + (det_sig[5] - det_sig[3])/(1+exp(det_sig[1]*(det_sig[7]+0.693147-dils2)))
SAMPLE200 <- det_sig[3] + (det_sig[5] - det_sig[3])/(1+exp(det_sig[1]*(det_sig[7]-0.693147-dils2)))
Xbend50l <- det_sig[7] + 0.693147-1.31696/det_sig[1]
Xbend200l <- det_sig[7] - 0.693147-1.31696/det_sig[1]
Xbend50u <- det_sig[7] + 0.693147+1.31696/det_sig[1]
Xbend200u <- det_sig[7] - 0.693147+1.31696/det_sig[1]
Xbend50 <- max(Xbend50l, Xbend50u)
Xbend200 <- min(Xbend200l, Xbend200u)
dummy <- plot_f(tab,sigmoid=NULL,det_sig=det_sig)
}
pl_df <- cbind(dils2, SAMPLE50, SAMPLE200)
#browser()
# scenario2
eqSpac <- abs((BPs[1]-BPs[2])/5)
optdils <- c((asyml+BPs[1])/2, BPs[1], BPs[1]+1*eqSpac, BPs[1]+2*eqSpac,BPs[1]+3*eqSpac,BPs[1]+4*eqSpac,BPs[2], (asymu+BPs[2])/2)
# scenario 3
eqSpac_3 <- abs((BPs[1]-BPs[2])/3)
optdils_3 <- c(BPs[1]-2*eqSpac_3, BPs[1]-eqSpac_3, BPs[1], BPs[1]+1*eqSpac_3, BPs[1]+2*eqSpac_3,BPs[2], BPs[2]+eqSpac_3, BPs[2]+2*eqSpac_3)
# scenario 6
Einh2 <- abs(((BPs[2]-BPs[1])*0.7)/5)
eqSpac2 <- (2*0.7/Einh)/3
optdils2 <- c((asyml+BPs[1])/2, BPs[1], EC50REF-1.5*Einh2, EC50REF-0.5*Einh2,EC50REF+0.5*Einh2,EC50REF+1.5*Einh2, BPs[2], (asymu+BPs[2])/2)
# steep slope
eqSpac3 <- (abs(Xbend200-Xbend50))/5
optdils3 <- c(Xbend200-eqSpac3,Xbend200, Xbend200+1*eqSpac3, Xbend200+2*eqSpac3,Xbend200+3*eqSpac3,Xbend200+4*eqSpac3,Xbend50, Xbend50+eqSpac3)
output$extremebps <- renderTable({
ExtremeBPs <- c(Xbend50,Xbend200)
DF2 <- data.frame(sample=c("50% sample (right)", "200% sample (left)"), Extreme_BPs=ExtremeBPs)
DF2
})
optD <- data.frame(cbind(optdils, optdils_3,optdils2, optdils3))
colnames(optD) <- c("scenario2","scenario3","scenario6","steep slope")
output$optimalDils <- renderTable({ optD })
output$adjlogdil <- renderTable({
adjlogdilfactors <- round(dilfactors,3)
adjlogdils <- round(dils2,3)
adjdils <- round(exp(dils2),3)
DilsTable <- data.frame('adjusted ln(dilutions)' = adjlogdils,
'adjusted ln_dilution_factors' = adjlogdilfactors,
'adjusted dilutions' = adjdils)
DilsTable
})
if (!is.null(Dat$p2)) {
p2 <- Dat$p2
p_dil <- p2 +
annotate("pointrange",x=dils2,y=rep(min_y, length(dils2)), xmin=min(dils2), xmax=max(dils2)) +
annotate("text", x=dils2,y=rep(min_y+(max_y-min_y)*0.05, length(dils2)), label=as.character(round(dils2,3))) +
annotate("text", x=dils2[-1]+(max(dils2)-min(dils2))*0.05,
y=rep(min_y+(max_y-min_y)*0.1, length(dils2[-1])),
label=as.character(round(dilfactors[-1],3))) +
geom_line(data=as.data.frame(pl_df),aes(x=dils2,y=SAMPLE50), color="grey15", linetype=2,
inherit.aes = F) +
geom_line(data=as.data.frame(pl_df),aes(x=dils2,y=SAMPLE200), color="grey15", linetype=2,
inherit.aes = F) +
geom_vline(xintercept=c(Xbend50,Xbend200), col="grey15", linetype=2) +
{if (input$scenario =="scenario 6") annotate("pointrange",x=optdils2,y=rep(min_y+(max_y-min_y)*0.2, length(optdils2)),
xmin=min(optdils2), xmax=max(optdils2), color="seagreen")} +
{if (input$scenario =="scenario 6") annotate("text",x=optdils2,y=rep(min_y+(max_y-min_y)*0.25, length(optdils2)),
label=as.character(round(optdils2,3)), color="seagreen")} +
{if (input$scenario =="scenario 2") annotate("pointrange",x=optdils,y=rep(min_y+(max_y-min_y)*0.2, length(optdils)),
xmin=min(optdils), xmax=max(optdils), color="seagreen")} +
{if (input$scenario =="scenario 2") annotate("text",x=optdils,y=rep(min_y+(max_y-min_y)*0.25, length(optdils)),
label=as.character(round(optdils,3)), color="seagreen")} +
{if (input$scenario =="scenario 3") annotate("pointrange",x=optdils_3,y=rep(min_y+(max_y-min_y)*0.2, length(optdils_3)),
xmin=min(optdils_3), xmax=max(optdils_3), color="seagreen")} +
{if (input$scenario =="scenario 3") annotate("text",x=optdils_3,y=rep(min_y+(max_y-min_y)*0.25, length(optdils_3)),
label=as.character(round(optdils_3,3)), color="seagreen")} +
{if (input$scenario =="steep slope") annotate("pointrange",x=optdils3,y=rep(min_y+(max_y-min_y)*0.2, length(optdils3)),
xmin=min(optdils3), xmax=max(optdils3), color="seagreen")} +
{if (input$scenario =="steep slope") annotate("text",x=optdils3,y=rep(min_y+(max_y-min_y)*0.25, length(optdils3)),
label=as.character(round(optdils3,3)), color="seagreen")} +
annotate("text",x=optdils[1],y=(max_y+min_y)*0.5,
label=paste("in green: optimal \n dilutions acc. to Whitepaper\n", input$scenario), color="seagreen",
size=14/.pt,fontface="bold")
}
print(p_dil)
})
#### Dilutions CI table ----
observe({
if (is.null(input$potencydiff)) return(NULL)
output$CIs <- renderTable({
PureErrFlag <- input$PureErr
if (is.null(Dat$coeffs_UN)) {
# checks if an EXCEL was uploaded
sigmoid <- sigmoid()
det_sig=NULL
ast = sigmoid()[1];bst = sigmoid()[5];cst = sigmoid()[7];dst = sigmoid()[3];ate = sigmoid()[2];
bte = sigmoid()[6];r_ = sigmoid()[8];
cte = cst-r_;dte = sigmoid()[4];
} else {
sigmoid <- NULL
det_sig <- Dat$coeffs_UN
ast <- det_sig[3]
ate <- det_sig[4]
bst <- det_sig[1]
bte <- det_sig[2]
cst <- det_sig[7]
cte <- det_sig[7] -log(input$potencydiff/100)
dst <- det_sig[5]
dte <- det_sig[6]
r_ <- log(input$potencydiff/100)
}
if (!is.na(input$NoDilSer)) {
noDilSer <- input$NoDilSer
} else if (!is.null(Dat$NoDilSeriesXL)) noDilSer <- Dat$noDilSeriesXL else noDilSer <- 3
if (!is.na(input$NoDil)) noDil <- input$NoDil else noDil <- length(Dat$newDils)
#browser()
tab <- Calc_DilRes(as=ast,at=ate,ds=dst,dt=dte,cs=cst,ct=cte,r=r_,bt=bte,bs=bst,
sd_fac=input$sdfac,log_conc=Dat$newDils,
# auslenkU=outlierU,
# auslenkM=outlierM,
# auslenkL=outlierL,
heteroNoise = FALSE, noDilSeries = noDilSer, noDils = noDil)
Limite <- list(as.numeric(input$lEACdiffla), as.numeric(input$uEACdiffla),
as.numeric(input$lEACratiola), as.numeric(input$uEACratiola),
as.numeric(input$lEACratioSlope), as.numeric(input$uEACratioSlope),
as.numeric(input$lEACratioua), as.numeric(input$uEACratioua),
as.numeric(input$lowerPot), as.numeric(input$upperPot),
as.numeric(input$lEACratioAdiff), as.numeric(input$uEACratioAdiff))
CItable <- tests_FUNC(tab,Limite,PureErrFlag=PureErrFlag)
CItable_ <- CItable[-c(1,2,6,8,9),-c(2,4,5)]
potAll <- pot4plFUNC(tab, input$PureErr)
restrPot <- potAll[1,1:4]
restrPot[2:4] <- round(as.numeric(restrPot[2:4]),5)
potAll_ <- rbind(CItable_, restrPot)
potAll_$CIwidth <- as.numeric(potAll_[,4])-as.numeric(potAll_[,3])
potAll_[,1] <- c("ratio of lower asymptotes","ratio of slopes","ratio of upper asymptotes", "ratio of asympt. differences","restricted potency")
output$bps <- renderTable({
DF <- data.frame(sample=names(Dat$bendpoints),BPs=Dat$bendpoints)
DF
})
return(potAll_)
})
})
#### simulations ----
observe({
observeEvent(input$goSim,{
sd_fac_ <- as.numeric(input$sdfac)
r_ <- log(as.numeric(input$potencydiff)/100)
Conc <- Dat$MetaConc
as = sigmoid()[1]; bs = sigmoid()[5];cs = sigmoid()[7];ds = sigmoid()[3];at = sigmoid()[2];
bt = sigmoid()[6];r = sigmoid()[8]; ct = cs-r_; dt = sigmoid()[4]
if (!is.null(Dat$MetaConc)) {
Conc <- Dat$MetaConc
} else {
Conc <- CONC()
}
log_dose <- log(Conc)
yAxfac <- (ds-as)
if (!is.na(input$NoDilSer)) {
noDilSer <- input$NoDilSer
} else if (!is.null(Dat$NoDilSeriesXL)) noDilSer <- Dat$noDilSeriesXL else noDilSer <- 3
if (!is.na(input$NoDil)) noDil <- input$NoDil else noDil <- length(Conc)
isRef <- rep(c(1,0),1,each=noDilSer*noDil)
isSample <- rep(c(0,1),1,each=noDilSer*noDil)
N <- as.numeric(input$simN)
av <- as*isRef + at*isSample + (ds*isRef + dt*isSample - as*isRef - at*isSample)/
(1+isRef*exp(bs*(cs - log_dose)) + isSample*exp(bt*(ct-log_dose)))
resHist <- matrix(NA,nrow=N, ncol=13)
residualsList <- list()
start.time2 <- Sys.time()
withProgress(message = 'Making plot', value=0, {
for (i in 1:N) {
if (input$heterosked) {
# heterosc noise
ro_jit <- matrix(unlist(map(av, function(x) x+rnorm(1,0,x*sd_fac_/100))), nrow=noDil, ncol=noDilSer*2)
} else {
# homosc noise
ro_jit <- matrix(unlist(map(av, function(x) x+rnorm(1,0,sd_fac_*yAxfac/100))), nrow=noDil, ncol=noDilSer*2)
}
# browser()
ro_jit <- abs(ro_jit)
ro_new <- cbind(ro_jit, log_dose)
all_l <- melt(data.frame(ro_new), id.vars="log_dose", variable.name = "replname", value.name = "readout")
all_l$isRef <- isRef
all_l$isSample <- isSample
all_l$Conc <- exp(all_l$log_dose)
pot <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(1,1,1,isSample))
potAll <- EDcomp(pot, percVec=c(50,50), interval="delta", display=FALSE)
potAll2 <- potAll[1:3]
RSS <- sum(pot$predres[,2]^2)
dfreed <- nrow(all_l)-5
MSE <- RSS/dfreed
potU <- drm(readout ~ Conc, isSample, data=all_l, fct=LL.4(names=c("b","d","a","c")),
pmodels=data.frame(isSample, isSample,isSample,isSample))
DF_U <- nrow(all_l)-8
uAsratio <- compParm(potU, "a",display=F)
uCIuAs <- uAsratio[1]+qt(0.975,DF_U)*uAsratio[2]
lCIuAs <- uAsratio[1]-qt(0.975,DF_U)*uAsratio[2]
lAsratio <- compParm(potU, "d",display=F)
uCIlAs <- lAsratio[1]+qt(0.975,DF_U)*lAsratio[2]
lCIlAs <- lAsratio[1]-qt(0.975,DF_U)*lAsratio[2]
Sloperatio <- compParm(potU, "b",display=F)
uCISlo <- Sloperatio[1]+qt(0.975,DF_U)*Sloperatio[2]
lCISlo <- Sloperatio[1]-qt(0.975,DF_U)*Sloperatio[2]
su <- summary(potU)
v <- vcov(potU)[c(5,6),c(5,6)]
Vd <- vcov(potU)[c(3,4),c(3,4)]
Va_d <- v+Vd
A_DTEST <- su$coefficients[6,1]-su$coefficients[4,1]
A_DREF <- su$coefficients[5,1]-su$coefficients[3,1]
if (abs(at/(sqrt(Va_d[2,2]/3))) > qt(0.95,2)) {
try(Fie_ad <- round(FiellerRatio(A_DREF,A_DTEST, Va_d),5))
}
if (!exists("Fie_ad")) Fie_ad <- NA
resHist[i,] <- c(potAll2, sqrt(MSE),Sloperatio[1],lCISlo, uCISlo,
uAsratio[1], lCIuAs, uCIuAs, Fie_ad[1],Fie_ad[2],Fie_ad[3])
colnames(resHist) <- c("pot4pl","lCI4pl","uCI4pl","RMSE","estSlope_ratio",
"lCISlope_ratio","uCISlope_ratio","estuAs_ratio",
"lCIuAs_ratio","uCIuAs_ratio","estAsyDiff_ratio",
"lCIAsyDiff_ratio", "uCIAsyDiff_ratio")
incProgress(1/N, detail=paste("Doing simulations",i))
} # withProgress
})
end.time2 <- Sys.time()
Dat$resHist <- resHist
})
})
#### simulation Histograms output ----
output$plotHistuAs <- renderPlot({
if (!is.null(Dat$resHist)) {
resHist <- Dat$resHist
#browser()
resHistuAs <- as.data.frame(resHist[,8:10])
resHistuAs_l <- melt(data.frame(resHistuAs), variable.name="ratio_CIs", value.name = "readout")
#browser()
lowquant_uAs <- quantile(resHistuAs[,2], probs=as.numeric(input$lowQuant)/100)
upquant_uAs <- quantile(resHistuAs[,3], probs=as.numeric(input$uppQuant)/100)
p_uAs <- ggplot(resHistuAs_l) +
geom_histogram(aes(readout, fill=ratio_CIs),alpha=0.5,position="identity") +
labs(title = paste("upper asymptote ratio EACs:", round(lowquant_uAs,3), " to ", round(upquant_uAs,3))) +
geom_vline(xintercept = c(lowquant_uAs, upquant_uAs), color="black", linetype="dashed", linewidth=1) +
geom_vline(xintercept = c(input$lEACratioua , input$uEACratioua), color="red", linetype="dashed", linewidth=1) +
theme_bw()
# asympt diff ratio
resHistAsDiff <- as.data.frame(resHist[,11:13])
resHistAsDiff_l <- melt(data.frame(resHistAsDiff), variable.name="ratio_CIs", value.name = "readout")
lowquant_AsDiff <- quantile(resHistAsDiff[,2], probs=as.numeric(input$lowQuant)/100)
upquant_AsDiff <- quantile(resHistAsDiff[,3], probs=as.numeric(input$uppQuant)/100)
p_AsDiff <- ggplot(resHistAsDiff_l, aes(readout, fill=ratio_CIs)) +
geom_histogram(alpha=0.5,position="identity") +
labs(title = paste("asymptote diff. ratio EACs:", round(lowquant_AsDiff,3), " to ", round(upquant_AsDiff,3))) +
geom_vline(xintercept = c(lowquant_AsDiff, upquant_AsDiff), color="black", linetype="dashed", linewidth=1) +
geom_vline(xintercept = c(input$lEACratioAdiff , input$uEACratioAdiff), color="red", linetype="dashed", linewidth=1) +
theme_bw()
# Slope ratio
resHistSlo <- as.data.frame(resHist[,5:7])
resHistSlo_l <- melt(data.frame(resHistSlo), variable.name="ratio_CIs", value.name = "readout")
lowquant_Slo <- quantile(resHistSlo[,2], probs=as.numeric(input$lowQuant)/100)
upquant_Slo <- quantile(resHistSlo[,3], probs=as.numeric(input$uppQuant)/100)
p_Slo <- ggplot(resHistSlo_l, aes(readout, fill=ratio_CIs)) +
geom_histogram(alpha=0.5,position="identity") +
labs(title = paste("Slope ratio EACs:", round(lowquant_Slo,3), " to ", round(upquant_Slo,3))) +
geom_vline(xintercept = c(lowquant_Slo, upquant_Slo), color="black", linetype="dashed", linewidth=1) +
geom_vline(xintercept = c(input$lEACratioSlope , input$uEACratioSlope), color="red", linetype="dashed", linewidth=1) +
theme_bw()
# poency ratio
resHistPot <- as.data.frame(resHist[,1:3])
resHistPot_l <- melt(data.frame(resHistPot), variable.name="ratio_CIs", value.name = "readout")
lowquant_Pot <- quantile(resHistPot[,2], probs=as.numeric(input$lowQuant)/100)
upquant_Pot <- quantile(resHistPot[,3], probs=as.numeric(input$uppQuant)/100)
#browser()
p_Pot <- ggplot(resHistPot_l, aes(readout, fill=ratio_CIs)) +
geom_histogram(alpha=0.5,position="identity") +
labs(title = paste("Poency ratio EACs:", round(lowquant_Pot,3), " to ", round(upquant_Pot,3))) +
geom_vline(xintercept = c(lowquant_Pot, upquant_Pot), color="black", linetype="dashed", linewidth=1) +
geom_vline(xintercept = c(input$lowerPot/100, input$upperPot/100), color="red", linetype="dashed", linewidth=1) +
theme_bw()
grid.arrange(p_Slo, p_AsDiff, p_uAs, p_Pot, nrow=1)
}
})
#### download XL report----
output$downloadXLReport <- downloadHandler(
filename= paste0("Report_4PLEvaluation", Dat$FileName,".pdf"),
content = function(file) {
tpdr <- tempdir()
tempReport <- file.path(tpdr,"Doc_BioassayReport.Rmd")
file.copy("Doc_BioassayReport.Rmd", tempReport, overwrite = T)
tempReportc <- file.path(tpdr,"logo.png")
file.copy("logo.png", tempReportc, overwrite = T)
rmarkdown::render(tempReport, output_file = file,
params = list(FileName = Dat$FileName,
author = Dat$author,
REP = REP,
coeffs = Dat$coeffs_UN),
envir = new.env(parent = globalenv()))
}
)
}
shinyApp(ui, server)